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pAW181

Plasmid

#113127

Purpose

E. coli expression vector for His-MBP-E. coli Cas1 R138A

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Insert

Cas1

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R138A

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAW210

Plasmid

#113189

Purpose

E. coli expression vector for untagged E. coli Cas1 H208A + Cas2 + CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

H208A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pVKG1-BSD

Plasmid

#108680

Purpose

Blasticidin resistant cassette for knock-in by VIKING method

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

SV40pro-BSD-polyA

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pWT029g

Plasmid

#96860

Purpose

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT029h

Plasmid

#96861

Purpose

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT049d

Plasmid

#96862

Purpose

guanine-agRNA (19 nt blocker with bulges, RFP activation)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (19 nt blocker with bulges, RFP activation)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT049e

Plasmid

#96863

Purpose

(d)guanine-agRNA (19 nt blocker with bulges, RFP activation)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine-agRNA (19 nt blocker with bulges, RFP activation)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016h

Plasmid

#96854

Purpose

HHR-bsgRNA-GFP (17 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

HHR-bsgRNA-GFP (17 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016d

Plasmid

#96855

Purpose

dHHR-bsgRNA-GFP (17 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

dHHR-bsgRNA-GFP (17 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016f

Plasmid

#96850

Purpose

HHR-bsgRNA-GFP (10 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

HHR-bsgRNA-GFP (10 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016e

Plasmid

#96851

Purpose

dHHR-bsgRNA-GFP (10 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

dHHR-bsgRNA-GFP (10 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016a

Plasmid

#96852

Purpose

HHR-bsgRNA-GFP (13 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

HHR-bsgRNA-GFP (13 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT016b

Plasmid

#96853

Purpose

dHHR-bsgRNA-GFP (13 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

dHHR-bsgRNA-GFP (13 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAP757-1

Plasmid

#99493

Purpose

6XHis::TEV::3XFlag::linker::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

6XHis::TEV::3XFlag::linker::Myc

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP605-1

Plasmid

#99482

Purpose

Myc::TEV::3XFlag::TEV::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

Myc::TEV::3XFlag::TEV::Myc

Expression

Bacterial

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAP686-1

Plasmid

#99491

Purpose

TEV::eGFP::ER::V5::linker::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::V5::linker::3XFlag

Expression

Bacterial

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAP640-2

Plasmid

#99487

Purpose

3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag

Expression

Bacterial

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3_DD_NvFP-7R_Ex2-LEFT

Plasmid

#90036

Purpose

TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

Depositor

Matthew Gibson

Article

Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

Insert

7R_Ex2-LEFT

Use

TALEN

Tags

3x Flag and Fok1-DD domain

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3_RR_NvFP-7R_Ex2-RIGHT

Plasmid

#90037

Purpose

TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

Depositor

Matthew Gibson

Article

Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

Insert

7R_Ex2-RIGHT

Use

TALEN

Tags

Fok1-RR domain and HA

Promoter

CMV

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pECT1B

Plasmid

#75447

Purpose

Plasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

pdt1B

Expression

Bacterial

Promoter

none

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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