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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,463 results for: 110

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Showing: 3361 - 3380 of 4463 results

  1. ATX3-JD

    Plasmid
    #184247
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Publication
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 Josephin domain (ATXN3 Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Premature stop codon V183X
    Promoter
    T7 promoter
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184247-plasmid-map-sequence-id-370313

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  2. ATX3-D1

    Plasmid
    #184246
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Publication
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 Josephin domain and UIM1 (ATXN3 Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Premature stop codon N263X
    Promoter
    T7 promoter
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184246-plasmid-map-sequence-id-370314

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  3. ATX3 13Q- I77K Q78K W87K

    Plasmid
    #185908
    Purpose
    Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).
    Depositor
    Sandra Macedo Ribeiro
    Publication
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Point mutations in Atx3 gene I77K Q78K and W87K
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185908-plasmid-map-sequence-id-370341

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  8. pChd4EosHaloTAF_Puro

    Plasmid
    #172099
    Purpose
    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    chromodomain helicase DNA binding protein 4 (Chd4 Synthetic, Mouse)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    172099-plasmid-map-sequence-id-338425

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  9. pDONR221-SLC35B1_STOP

    Plasmid
    #161265
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC35B1 (SLC35B1 Human)
    Expression
    Mammalian
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    161265-plasmid-map-sequence-id-479342

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  14. pTSHZ1-donor

    Plasmid
    #132479
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TSHZ1 (TSHZ1 Human)
    Use
    CRISPR
    Tags
    GFP
    Mutation
    none
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    132479-plasmid-map-sequence-id-274870

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  20. p667-UBC-TLK2-V5-miniTurbo_IDG-K

    Plasmid
    #135249
    Purpose
    Gateway destination clone of TLK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TLK2-V5-miniTurbo (TLK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135249-plasmid-map-sequence-id-268559

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Showing: 3361 - 3380 of 4463 results