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GFP-ataxin-3 *SIM

Plasmid

#89979

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutated 162IFVV to 162AFAA

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ETFB_WT_V5

Plasmid

#82987

Purpose

Gateway Donor vector containing ETFB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ETFB (ETFB Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CHD2_p.P474R

Plasmid

#81474

Purpose

Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CHD2 (CHD2 Human)

Use

Gateway Cloning

Mutation

P474R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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IDP254

Plasmid

#87004

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

postCLAP

Use

Synthetic Biology

Tags

spGFP (1-10)

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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DGKQ gRNA (BRDN0001145367)

Plasmid

#77378

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKQ

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKQ (DGKQ Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DGKQ gRNA (BRDN0001162263)

Plasmid

#77379

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKQ

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKQ (DGKQ Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DGKQ gRNA (BRDN0001146605)

Plasmid

#77380

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKQ

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKQ (DGKQ Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BCR gRNA (BRDN0001148847)

Plasmid

#76907

Purpose

3rd generation lentiviral gRNA plasmid targeting human BCR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BCR (BCR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BCR gRNA (BRDN0001144903)

Plasmid

#76909

Purpose

3rd generation lentiviral gRNA plasmid targeting human BCR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BCR (BCR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AMHR2 gRNA (BRDN0001149470)

Plasmid

#76657

Purpose

3rd generation lentiviral gRNA plasmid targeting human AMHR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AMHR2 (AMHR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AMHR2 gRNA (BRDN0001149245)

Plasmid

#76659

Purpose

3rd generation lentiviral gRNA plasmid targeting human AMHR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AMHR2 (AMHR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AMHR2 gRNA (BRDN0001148442)

Plasmid

#76660

Purpose

3rd generation lentiviral gRNA plasmid targeting human AMHR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AMHR2 (AMHR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIM2 gRNA (BRDN0001149489)

Plasmid

#76384

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIM2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIM2 (PIM2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIM2 gRNA (BRDN0001147820)

Plasmid

#76385

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIM2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIM2 (PIM2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TLK2 gRNA (BRDN0001146448)

Plasmid

#75615

Purpose

3rd generation lentiviral gRNA plasmid targeting human TLK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TLK2 (TLK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TLK2 gRNA (BRDN0001147069)

Plasmid

#75616

Purpose

3rd generation lentiviral gRNA plasmid targeting human TLK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TLK2 (TLK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TLK2 gRNA (BRDN0001147939)

Plasmid

#75617

Purpose

3rd generation lentiviral gRNA plasmid targeting human TLK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TLK2 (TLK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BCR gRNA (BRDN0001148322)

Plasmid

#76908

Purpose

3rd generation lentiviral gRNA plasmid targeting human BCR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BCR (BCR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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AMHR2 gRNA (BRDN0001147673)

Plasmid

#76658

Purpose

3rd generation lentiviral gRNA plasmid targeting human AMHR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AMHR2 (AMHR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-RIPK3 V460P

Plasmid

#78826

Purpose

To detect localization of RIPK3 V460P protein in live mammalian cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

V460P

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only