We narrowed to 7,211 results for: CIT

1. pHH0103_ARHGEF9-1/1

   Plasmid

   #91417

   Purpose

   Protein expression and purification of human SH3 domain construct ARHGEF9-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ARHGEF9-1/1 (ARHGEF9 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-dSpCas9-HF1-VP64-6xHis

   Plasmid

   #92118

   Purpose

   Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   T7

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLEX307-SARS-CoV-3CL-C145A

   Plasmid

   #160281

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV 3CL protease with C145A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   C145A

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTE4999

   Plasmid

   #107555

   Purpose

   Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(dead1)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   D917A

   Promoter

   CMV

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GST-TEV-FUS-1-373aa

   Plasmid

   #29631

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS-1-373aa (FUS Human)
   

   Use

   Tags

   GST and TEV

   Expression

   Bacterial

   Mutation

   Contains FUS aa# 1-373

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-Syn-CsChR-GFP

   Plasmid

   #58841

   Purpose

   AAV expression of CsChR-GFP under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   CsChR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GST-TEV-FUS-267-526aa

   Plasmid

   #29630

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS-267-526aa (FUS Human)
   

   Use

   Tags

   GST and TEV

   Expression

   Bacterial

   Mutation

   Contains FUS aa# 267-526

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

8. 423Gal-FUS-YFP

   Plasmid

   #29615

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

9. LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode

   Plasmid

   #170222

   Purpose

   Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   R24C

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid

    #92102

    Purpose

    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PARD6A_PDZ_1

    Plasmid

    #103946

    Purpose

    Protein expression and purification of TAX INTERACTION PROTEIN PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PARD6A (PARD6A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Pdpy-30-miniCAFE

    Plasmid

    #170115

    Purpose

    Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    MiniCAFE
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D8A, HNH-truncation (Δ495–609 aa) in CjCas9

    Promoter

    Dpy-30

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLEX307-MERS-CoV-3CL-C148A

    Plasmid

    #160283

    Purpose

    Allows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C148A

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDestTol2-actinb-kcnj13-EGFP

    Plasmid

    #164940

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    EGFP

    Expression

    Mutation

    Stop codon deleted

    Promoter

    actinb (actb2) promoter

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_ARHGEF6-1/1

    Plasmid

    #91515

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF6-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF6-1/1 (ARHGEF6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLEX307-IBV-3CL-C143A

    Plasmid

    #160289

    Purpose

    Allows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C143A

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTE4565

    Plasmid

    #88903

    Purpose

    Expresses inactive/dead, humanized MbCpf1 nuclease

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(D986A)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GST-TEV-FUS-1-413aa

    Plasmid

    #29632

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS-1-413aa (FUS Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Bacterial

    Mutation

    Contains FUS aa# 1-413

    Promoter

    Available since

    July 18, 2011

    Availability

    Academic Institutions and Nonprofits only

19. LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode

    Plasmid

    #170217

    Purpose

    Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CASP8 (CASP8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    C360A

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DSPQ-HTT-TALEN2 BSD

    Plasmid

    #92246

    Purpose

    TALEN targeting downstream of HTT gene (ELD) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN1 ZEO. TALEN: NN, HD, NN, NN, HD, NG, NN, NI, NN, NN, HD, NI, NN, HD, NI, NN

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge