We narrowed to 30,287 results for: jun

1. pGFP-HBx NESM

   Plasmid

   #24932

   Depositor

   Xin Wang

   Article

   Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

   Insert

   HBx
   

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   L98A,G99A,L100A

   Available Since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. p221z-AtMIR390a

   Plasmid

   #118388

   Purpose

   Entry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   AtMIR390a backbone
   

   Use

   RNAi

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFBXC3GH

   Plasmid

   #49051

   Purpose

   FX cloning insect cell expression vector

   Depositor

   Markus Gruetter

   Article

   Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

   Type

   Empty backbone

   Tags

   3C protease site (PreScission site), GFP, and dec…

   Expression

   Insect

   Promoter

   polH (polyhedrin)

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_Lifeact_STf

   Plasmid

   #182011

   Purpose

   CMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   Lifeact_STf
   

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6-2 SNAP-Syk tSH2

   Plasmid

   #113028

   Purpose

   for purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   SNAP-Syk tSH2
   

   Tags

   SNAP

   Expression

   Bacterial

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PH-Gab2-GFP

   Plasmid

   #35147

   Depositor

   Sergio Grinstein

   Article

   Gu et al J Cell Biol. 2003 Jun 23;161(6):1151-61.

   Insert

   Gab2 PH Domain(x2)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains two copies of the PH domain of Gab2: aa …

   Promoter

   CMV

   Available Since

   March 20, 2012

   Availability

   Academic Institutions and Nonprofits only

7. Actinin-C-stFRET

   Plasmid

   #61104

   Purpose

   Expression of α-actinin with C-term stFRET inserted to measure mechanical stress in real time

   Depositor

   Fred Sachs

   Article

   Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

   Insert

   α-actinin-stFRET
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRPML2-MYC

   Plasmid

   #18829

   Depositor

   Craig Montell

   Article

   Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

   Insert

   TRPML2 (Mcoln2 Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

9. Halo-Erk2 donor

   Plasmid

   #113104

   Purpose

   Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   N terminal Erk2 donor region including homology arms and HaloTag
   

   Use

   Synthetic Biology

   Available Since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cas12c-sgRNA_BbsI_CAM

    Plasmid

    #183221

    Purpose

    Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c single guide RNA containing BbsI sites in spacer sequence
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-SUMO mCER-Prx2

    Plasmid

    #158226

    Purpose

    Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression)

    Depositor

    Tobias Dick

    Article

    Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

    Insert

    mCER-Prx2
    

    Expression

    Bacterial

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pF3BGX-TangoL-lexA

    Plasmid

    #138404

    Purpose

    RMCE vector for generating C-terminal Tango fusion

    Depositor

    Shu Kondo

    Article

    Katow et al J Neurogenet. 2019 Mar - Jun;33(2):44-51

    Insert

    TEVcs(ENLYFQL)-LexAp65
    

    Expression

    Insect

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPEE17

    Plasmid

    #128361

    Purpose

    Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.

    Depositor

    James Fraser

    Article

    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

    Insert

    Acetamidase (amdS)
    

    Use

    Unspecified

    Promoter

    TEF1

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. FRB-polySH33-EGFP

    Plasmid

    #175792

    Purpose

    Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FRB-polySH35

    Plasmid

    #175234

    Purpose

    Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCM-HA-Gas1

    Plasmid

    #38313

    Depositor

    Susan Lindquist

    Article

    McLellan et al ACS Chem Biol. 2012 Jun 22.

    Insert

    Gas1 (GAS1 Budding Yeast)
    

    Tags

    2xHA

    Mutation

    contains promoter region

    Promoter

    Endogenous

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac GST-Plk4

    Plasmid

    #80264

    Purpose

    baculovirus creation plasmid for GST-Plk4

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Tags

    GST

    Expression

    Insect

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1B-EGFP-DmDCP1

    Plasmid

    #21684

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

    Insert

    Decapping protein 1 (Dcp1 Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmMaple2-CAM

    Plasmid

    #101149

    Purpose

    Fluorescent protein for superresolution imaging

    Depositor

    Xiaowei Zhuang

    Article

    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

    Insert

    E. coli codon optimized mMaple2
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

    Plasmid

    #183077

    Purpose

    Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c tracrRNA and crRNA ending with a HDV ribozyme
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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