We narrowed to 54,151 results for: tra

1. pCMV/myc/ER-αT9ib

   Plasmid

   #110768

   Purpose

   Expresses ER intrabody against mouse and human TLR9

   Depositor

   Thomas Böldicke

   Article

   Reimer et al Cell Mol Biol Lett. 2013 Sep;18(3):433-4

   Insert

   intrabody against mouse and human TLR9
   

   Use

   Tags

   ER retention signal (SEKDEL), ER signal peptide, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF4 CMV 5TOP intron renilla TRICK CTE polyA

   Plasmid

   #64544

   Purpose

   5′ TOP TRICK reporter mRNA

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   12x PP7 stem-loops
   

   Use

   Tags

   24xMS2 stem loops, 5' TOP (5′ terminal oligo…

   Expression

   Mammalian

   Mutation

   Promoter

   Tetracycline-inducible promoter and 5′ UTR of hum…

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension

   Plasmid

   #176542

   Purpose

   Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Inserts

   MBP tag
   TnpB
   

   Use

   Tags

   3' extension derived from endogenous locus a…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TfR-moxGFP-DHFR

   Plasmid

   #173012

   Purpose

   For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   TfR-moxGFP-DHFR
   

   Use

   Tags

   DHFR and moxGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA

   Plasmid

   #176538

   Purpose

   Mammalian expression of nuclear localized AwaIscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   AwaIscB
   

   Use

   Tags

   Nucleoplasmin NLS-3xHA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB116 - pL2_pSB90_fLUC-I_P19

   Plasmid

   #123191

   Purpose

   binary plant vector for transient TBSV P19 and fLUC (with intron) expression

   Depositor

   Carolyn Lee-Parsons, Erin Cram

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   2x35S::P19::tNOS 2x35S::fLUC-I::tNOS
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB118 - pL2_pSB90_fLUC-I_HcPro

   Plasmid

   #123193

   Purpose

   binary plant vector for transient TEV HcPro and fLUC (with intron) expression

   Depositor

   Carolyn Lee-Parsons, Erin Cram

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_GW_Dest

   Plasmid

   #86955

   Purpose

   Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.

   Depositor

   Joseph Dougherty

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-WIV1-CoV

   Plasmid

   #164438

   Purpose

   Encodes WIV1-CoV Spike Protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   WIV1-CoV Spike
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRCPam

    Plasmid

    #195861

    Purpose

    Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.

    Depositor

    Gregory Phillips

    Article

    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

    Insert

    Amber Chromogenic Protein on pRCPam plasmid
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gln62X

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBJG1 T7-8xHis-OGT D554N

    Plasmid

    #154279

    Purpose

    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

    Depositor

    Suzanne Walker

    Article

    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Tags

    8x His, HRV3C protease site, and T7 leader

    Expression

    Bacterial

    Mutation

    Aspartate 554 changed to Asparagine- bacterial co…

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII

    Plasmid

    #209292

    Purpose

    Expresses R2Bm-Cas9(H840A) fusion in E. coli

    Depositor

    Feng Zhang

    Article

    Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

    Insert

    R2
    

    Use

    Tags

    His, MBP, SpCas9(H840A), StrepII, and bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-SOCS6-WT

    Plasmid

    #74496

    Purpose

    Overexpression of SOCS6 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

    Insert

    SOCS6 (Socs6 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    unknown

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pRSETC-RdgBbeta-splice variant 1

    Plasmid

    #58276

    Purpose

    bacterial expression of human RdgBbeta splice variant 1

    Depositor

    Shamshad Cockcroft

    Article

    Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

    Insert

    RdgBbeta (PITPNC1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pET28a-baPrs-hdNadV

    Plasmid

    #91950

    Purpose

    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

    Inserts

    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Leucine 135 to Isoleucine

    Promoter

    T7

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLPCX-Cx43-FS-IRES-GFP

    Plasmid

    #65438

    Purpose

    Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    Cx43 (GJA1 Human)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    frameshift (FS) mutation 35delA

    Promoter

    CMV

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCHOP10(dLZ).pCDNA1

    Plasmid

    #21900

    Depositor

    David Ron

    Article

    Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

    Insert

    CHOP10 (Ddit3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The coding region of the murine CHOP-10 cDNA was …

    Promoter

    Available since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pKS7107

    Plasmid

    #89051

    Purpose

    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Nm crRNA
    Nm tracrRNA
    hNmCas9
    

    Use

    CRISPR

    Tags

    HA tag, NLS, and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSB115 - pL2_pSB90_fLUC-I_tGFP-I

    Plasmid

    #123190

    Purpose

    binary plant vector for transient tGFP (with intron) and fLUC (with intron) expression

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF1304

    Plasmid

    #144293

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZMIZ2 (ZMIZ2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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