We narrowed to 10,301 results for: yeast expression

1. pML107-VHT1

   Plasmid

   #232898

   Purpose

   Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   VHT1 gRNA (VHT1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pML107-ADE13

   Plasmid

   #232887

   Purpose

   Plasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   ADE13 gRNA (ADE13 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pML107-LAS17

   Plasmid

   #232892

   Purpose

   Plasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   LAS17 gRNA (LAS17 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pML104-LEU2-2

   Plasmid

   #232883

   Purpose

   Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   LEU2 gRNA (LEU2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pML107-INO80

   Plasmid

   #232890

   Purpose

   Plasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   INO80 gRNA (INO80 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TINGL pDRF1

   Plasmid

   #226436

   Purpose

   Expresses TINGL (mTq2-based glucose sensor) in yeast

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2024.11.29.626064

   Insert

   TINGL
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PMA1

   Available Since

   Dec. 2, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pRS415 GPDpro-RNQ1-CFP

   Plasmid

   #224887

   Purpose

   Low copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).

   Depositor

   Paul Herman

   Insert

   RNQ1 (RNQ1 Budding Yeast)
   

   Use

   Tags

   CFP

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p416-GPD-HA-PPK(mutant)

   Plasmid

   #183944

   Purpose

   Expresses HA-tagged catalytic mutant of E. coli PPK from yeast GPD promoter

   Depositor

   Michael Downey

   Insert

   PPK(mutant) (ppk E. coli)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   mutations in His435, His454, and His592 to Alanine

   Promoter

   GPD

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNSU299_MET16

   Plasmid

   #166091

   Purpose

   Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   MET16 (MET16 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available Since

   May 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNSU299_CPR1_1

    Plasmid

    #166074

    Purpose

    Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    CPR1 (CPR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNSU299_GAL7

    Plasmid

    #166089

    Purpose

    Plasmid for constituive spCas9 and tet-inducible GAL7 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    GAL7 (GAL7 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNSU299_GLK1

    Plasmid

    #166090

    Purpose

    Plasmid for constituive spCas9 and tet-inducible GLK1 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    GLK1 (GLK1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNSU299_LHS1

    Plasmid

    #166092

    Purpose

    Plasmid for constituive spCas9 and tet-inducible LHS1 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    LHS1 (LHS1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNSU299_YBL055C

    Plasmid

    #166077

    Purpose

    Plasmid for constituive spCas9 and tet-inducible YBL055C targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    YBL055C (near PTC3) (YBL055C Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNSU299_SLA1

    Plasmid

    #166075

    Purpose

    Plasmid for constituive spCas9 and tet-inducible SLA1 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    SLA1 (SLA1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNSU299_PDR12

    Plasmid

    #166093

    Purpose

    Plasmid for constituive spCas9 and tet-inducible PDR12 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    PDR12 (PDR12 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNSU299_TDH2

    Plasmid

    #166082

    Purpose

    Plasmid for constituive spCas9 and tet-inducible TDH2 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    TDH2 (TDH2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNSU299_LYS9

    Plasmid

    #166085

    Purpose

    Plasmid for constituive spCas9 and tet-inducible LYS9 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    LYS9 (LYS9 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pC1-CMV-mito-DAAO

    Plasmid

    #141132

    Purpose

    Expresses DAAO with mitochondrial localization signal in mammalian cells

    Depositor

    Vsevolod Belousov

    Insert

    mito-DAAO
    

    Use

    Tags

    2 mitochondrial localization signals and 7xHis

    Expression

    Mammalian

    Mutation

    deleted DAAO amino acids 367-368

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTH761-CEN-mCherry_v4

    Plasmid

    #40599

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    mCherry ORF partially codon optimised for yeast e…

    Promoter

    GPD (TDH3)

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge