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rEcoli XpS

Bacterial Strain

#192872

Purpose

MG1655-C321 -- All 321 UAG codons changed to UAA

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Bacterial Resistance

Bleocin (Zeocin)

Species

Escherichia coli

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
pU6-hL1-5'_3.3kb plasmid

Plasmid

#226006

Purpose

Plasmid used to generate DNA-FISH probe targeting at human L1HS

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

Human L1 element 3.3kb sequences at the 5' end

Use

Synthetic Biology

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-mini v2-HF24_F5

Plasmid

#195719

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#5 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pN1-HsSmg5-V5-SBP-MBP_V

Plasmid

#147768

Purpose

Mammalian Expression of HsSmg5

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3D19-4/5

Plasmid

#91426

Purpose

Protein expression and purification of human SH3 domain construct SH3D19-4/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D19-4/5 (SH3D19 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3D19-3/5

Plasmid

#91381

Purpose

Protein expression and purification of human SH3 domain construct SH3D19-3/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D19-3/5 (SH3D19 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3D19-1/5

Plasmid

#91339

Purpose

Protein expression and purification of human SH3 domain construct SH3D19-1/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D19-1/5 (SH3D19 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3D19-2/5

Plasmid

#91272

Purpose

Protein expression and purification of human SH3 domain construct SH3D19-2/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D19-2/5 (SH3D19 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pPN263

Plasmid

#91608

Purpose

Express sgRNA targeting human EPHX2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

EPHX2 (EPHX2 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

Plasmid

#112669

Purpose

Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.

Depositor

Catherine Verfaillie

Article

Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

Insert

tdT

Use

AAV; Donor plasmid for recombinase-mediated casse…

Expression

Mammalian

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN1-1/5-IS2/3

Plasmid

#91318

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-1/5-IS2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-1/5-IS2/3 (ITSN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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PUC57_BAG3wt-eGFP

Plasmid

#98182

Purpose

Codes for full length, GFP tagged, human wildtype Bag3

Depositor

Robert Bryson-Richardson

Article

Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

Insert

BAG3wt-eGFP (BAG3 Human)

Tags

GFP

Expression

Bacterial

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT2-14-GFP11_SEPT2-14-GFP10

Plasmid

#180350

Purpose

mammalian co-expression of human SEPT2 fused to GFP10 and of human SEPT2 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN2 (SEPTIN2 Human)
SEPTIN 2 (SEPTIN2 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pNIC28-Bsa4_ZV-NS5-RdRp

Plasmid

#179847

Purpose

The RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89

Depositor

Julien Lescar

Article

Gharbi-Ayachi et al J Virol. 2020 Oct 14;94(21). pii: JVI.00

Insert

Zika Virus Non-structural protein 5 RdRp domain

Tags

6-histine

Expression

Bacterial

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT2 NCmut-14-GFP11_SEPT2 NCmut-14-GFP10

Plasmid

#180357

Purpose

mammalian co-expression of human SEPT2 NCmut fused to GFP10 and of human SEPT2 NCmut fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN2 (SEPTIN2 Human)
SEPTIN 2 (SEPTIN2 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT2 F20D, V27D

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCMVNeoDEST(705-1)-hAXL

Plasmid

#124796

Purpose

Expresses AXL in mammalian cells

Depositor

Kenneth Pienta

Article

Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

Insert

AXL (AXL Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-L

Plasmid

#182279

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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Stx5S-mCherry

Plasmid

#165087

Purpose

Expresses Stx5S-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Stx5 (Short isoform) (STX5 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Lacks amino acids 1-54, enocdes short isoform of …

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-H

Plasmid

#182281

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-H (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCM3.19

Plasmid

#19401

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

tbb-2 ORF+3' in pDONR201 (tbb-2 Nematode)

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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