We narrowed to 1,995 results for: Ipp

1. pPHR-EYFP-hPMLIII

   Plasmid

   #103825

   Purpose

   Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-EYFP-hPMLIII (CRY2, PML Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIBN-TetR-tagRFP-T

   Plasmid

   #103809

   Purpose

   'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-TetR-tagRFP-T (CIB1, tetR E. coli, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TetR: A4G (M2V)

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. TVBB C-term-Dendra2

   Plasmid

   #169216

   Purpose

   Targeting vector backbone to support a knock-in of Linker-Dendra2 at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked Dendra2-P2A-Puro
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPHR-EYFP-NLS-VP16

   Plasmid

   #103822

   Purpose

   Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-EYFP-VP16 (CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   VP16: G126T (silent, G42G)

   Promoter

   CMV

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCIG-LRRC37B

   Plasmid

   #220790

   Purpose

   To express the human receptor LRRC37B in mammalian cells together with EGFP

   Depositor

   Pierre Vanderhaeghen

   Article

   Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

   Insert

   LRRC37B (LRRC37B Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PHR-GFP-VP16

   Plasmid

   #183927

   Purpose

   Optogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposure

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   PHR-GFP-VP16 (CRY2, UL48 Synthetic, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PHR-GFP-FUSN-VP16

   Plasmid

   #183933

   Purpose

   Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   PHR-GFP-FUSN-VP16 (CRY2, UL48 Synthetic, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mEmerald-Sec61b-C1

   Plasmid

   #90992

   Purpose

   Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

   Insert

   mEmerald-Sec61b (SEC61B Human, Synthetic)
   

   Use

   Tags

   mEmerald (green fluorescence)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCherry-Sec61b-C1

   Plasmid

   #90994

   Purpose

   Expresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

   Insert

   mCherry-Sec61b (SEC61B Human, Synthetic)
   

   Use

   Tags

   mCherry (red fluorescence)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mApple-Sec61b-C1

    Plasmid

    #90993

    Purpose

    Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mApple-Sec61b (SEC61B Human, Synthetic)
    

    Use

    Tags

    mApple (red fluorescence)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEmerald-GPAT4152-208

    Plasmid

    #134468

    Purpose

    GPAT4152-208 expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    GPAT4 (GPAT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GPAT4, amino acids 152-208

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

    Plasmid

    #182424

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
    VNp-LZ
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp), and Vesicle Nuc…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TVBB C-term-mScarlet-Blast

    Plasmid

    #169221

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet-P2A-Blast
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TVBB C-term-mScarlet-Puro

    Plasmid

    #169220

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet-P2A-Puro
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TVBB C-term-mNeongreen-Puro

    Plasmid

    #169228

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen-P2A-Puro
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV 4xSTAR-mTagBFP2-blast

    Plasmid

    #125239

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mTagBFP2 (codon optimized)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Tol2 8xSTAR-mNeonGreen. PGK-puro

    Plasmid

    #136255

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TVBB C-term-mNeongreen-Blast

    Plasmid

    #169229

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen-P2A-Blast
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Halo-p58

    Plasmid

    #166896

    Purpose

    mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    p58 (Lman1 Rat)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    L405Q, R419S- please see depositor comments

    Promoter

    CMV

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TVBB C-term-mTurquoise2-Hygro

    Plasmid

    #169225

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mTurquoise2-P2A-Hygro
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge