-
Plasmid#193721PurposeOptogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transportDepositorInserthsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Synthetic, Human)
UseTagsmVenus-SSPB(micro)ExpressionMammalianMutationhsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …PromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_K490_GFP_SNAP
Plasmid#159721PurposeExpresses kinesin-1 (amino acids 1-490) in Sf9 cells with a C-terminal GFP and SNAP tagDepositorInsertKIF5B (only amino acids 1-490), K490 (KIF5B Human)
UseTagsGFP and SNAP tags and ZZ affinity tagExpressionInsectMutationOnly amino acids (1-490)PromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PTCH1-D894N
Plasmid#116611PurposeLentiviral expression of PTCH1 D894NDepositorInsertPTCH1 (PTCH1 Human)
UseLentiviralTagsExpressionMutationD894NPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PTCH1-G1212D
Plasmid#116612PurposeLentiviral expression of PTCH1 G1212DDepositorInsertPTCH1 (PTCH1 Human)
UseLentiviralTagsExpressionMutationG1212DPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PTCH1-R1308Kfs*64
Plasmid#116613PurposeLentiviral expression of PTCH1 R1308Kfs*64DepositorInsertPTCH1 (PTCH1 Human)
UseLentiviralTagsExpressionMutationR1308Kfs*64PromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PTCH1-S1203Rfs*52
Plasmid#116614PurposeLentiviral expression of PTCH1 S1203Rfs*52DepositorInsertPTCH1 (PTCH1 Human)
UseLentiviralTagsExpressionMutationS1203Rfs*52PromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PTCH1-T768I
Plasmid#116616PurposeLentiviral expression of PTCH1 T768IDepositorInsertPTCH1 (PTCH1 Human)
UseLentiviralTagsExpressionMutationT768IPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAPK1 gRNA (BRDN0001149093)
Plasmid#77788Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK1DepositorInsertMAPK1 (MAPK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK1 gRNA (BRDN0001147355)
Plasmid#77787Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK1DepositorInsertMAPK1 (MAPK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIRPA-Fc(DAPA)-AviTag-6xHis
Plasmid#156783PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSIRPA (SIRPA Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa-FRB-3myc-KIF1A tail 391-1698
Plasmid#64286PurposeKIF1A aa 391-1698 with N-terminal FRB and triple myc tagsDepositorUseTags3myc and FRBExpressionMammalianMutationaa 391-1698, 5'end mostly matches rat, 3…Promoterchicken Beta ActinAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
shERK2b-mlpx-puro
Plasmid#65230Purposeencodes a shRNA against ERK2DepositorInsertshRNA against ERK2 (MAPK1 Human)
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAPK1 gRNA (BRDN0001147498)
Plasmid#77789Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK1DepositorInsertMAPK1 (MAPK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSN879
Plasmid#190693PurposeKnockdown mouse KIF5A, KIF5B and KIF5C simultaneously. mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A).DepositorUseRNAiTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
shERK2a-mlpx-puro
Plasmid#65229Purposeencodes a shRNA against ERK2DepositorInsertshRNA against ERK2 (MAPK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
shERK1a-mlpx-puro
Plasmid#65228Purposeencodes a shRNA against ERK1DepositorInsertshRNA against ERK2 (MAPK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
ERK1C202
Plasmid#164647PurposeBacterial expression plasmid for His6-ERK1C202DepositorInsertERK1C202 (MAPK1 Human)
UseTagsHis6-tagExpressionBacterialMutationK71R/C82S/C144S/C178S/C183S/T202C/C271SPromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
shERK2b-mlpx-neo
Plasmid#65231Purposeencodes a shRNA against ERK2DepositorInsertshRNA against ERK2 (MAPK1 Human)
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
ERK1C82C202
Plasmid#164650PurposeBacterial expression plasmid for His6-ERK1C82C202DepositorInsertERK1C82C202 (MAPK1 Human)
UseTagsHis6-tagExpressionBacterialMutationK71R/C144S/C178S/C183S/T202C/C271SPromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
MK01_HUMAN_D0
Plasmid#79713PurposeThis plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK01 (MAPK1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only