We narrowed to 858 results for: PX330

1. pX330-NQL005-SOX2-sgRNA

   Plasmid

   #175553

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-NLC-DHFR-SpCas9

   Plasmid

   #124523

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NLC-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAID-CMV linearizing in pX330

   Plasmid

   #140609

   Purpose

   Crispr/Cas9 plasmid for linearization of pAID-CMV

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   sgRNA for pAID-EF1a plasmid digestion
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-Flag-eSpCas9-D10A

   Plasmid

   #80449

   Purpose

   enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   ehSpCas9_D10A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330(puro) Rosa26-H3F3B

   Plasmid

   #73131

   Purpose

   Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

   Depositor

   Agnel Sfeir

   Article

   Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

   Insert

   spCas9
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-Flag-eSpCas9-H840A

   Plasmid

   #80454

   Purpose

   enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   ehSpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-NQL007-NANOG-sgRNA

   Plasmid

   #175555

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330 Mouse 5' Npm1 gRNA

   Plasmid

   #127900

   Purpose

   WT Cas9 Vector targeting the 5' end of the mouse Npm1 gene

   Depositor

   Mark Groudine

   Insert

   gRNA/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-HeFSpCas9-H840A

   Plasmid

   #80456

   Purpose

   high fidelity enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   HeFSpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-U6-MYC-Guide-hSpCas9

    Plasmid

    #228574

    Purpose

    Expression of human MYC gRNA; CRISPR mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    MYC gRNA (MYC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-U6-E690_guide-CBh-hSpCas9

    Plasmid

    #188545

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-U6-W308_guide-CBh-hSpCas9

    Plasmid

    #188546

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-U6-L101_guide-CBh-hSpCas9

    Plasmid

    #188547

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330 NLC-FKBP-Cas9

    Plasmid

    #162726

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330 Mouse 3' Ubf gRNA

    Plasmid

    #127902

    Purpose

    WT Cas9 Vector targeting the 3' end of the mouse Ubf gene

    Depositor

    Mark Groudine

    Insert

    gRNA for Mouse 3' Ubf
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. px330 Gatad2a sgRNA (for deletion)

    Plasmid

    #110814

    Purpose

    Expressed sgRNA for generating Gatad2a deletion (knockout ) in mouse cell lines

    Depositor

    Jacob Hanna

    Article

    Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

    Insert

    mGataD2a (Gatad2a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-Flag-HFSpCas9-H840A

    Plasmid

    #80455

    Purpose

    high fidelity SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    HFSpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Intron-Tagging-pX330-Cas9-Blast

    Plasmid

    #159741

    Purpose

    Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    donor plasmid targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. plenti-px330-CRBN-T1-pGK-Pur

    Plasmid

    #107382

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. px330 Rosa26 AscI LH291Gu1 (LH500)

    Plasmid

    #99628

    Purpose

    Plasmid used to target the pre-modified Rosa26 allele with Cas9

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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