We narrowed to 5,324 results for: cycs

1. FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

   Plasmid

   #58442

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

   Plasmid

   #223065

   Purpose

   Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
   

   Use

   CRISPR

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Mutation

   Three regions of SpCas9 encode type IIS restricti…

   Promoter

   Dual T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

   Plasmid

   #110547

   Purpose

   CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.

   Depositor

   Ichiro Matsumura

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   pBAD

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 FRT/TO/STRP 3x FLAG cyclin B1 L45A (1391)

   Plasmid

   #61841

   Purpose

   mammalian expression of cyclin B1 non-degradable L45A mutant

   Depositor

   Jonathon Pines

   Article

   Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

   Insert

   cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   2xSTREP and 3xFLAG

   Expression

   Mammalian

   Mutation

   L45A

   Promoter

   CMV

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

5. FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

   Plasmid

   #58440

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

   Plasmid

   #58439

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

   Plasmid

   #58441

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 3 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. -962 human cyclin D1 promoter AP-1 site mutant pGL3Basic

   Plasmid

   #32728

   Depositor

   Frank McCormick

   Article

   Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

   Insert

   CCND1 promoter (CCND1 Human)
   

   Use

   Luciferase; Luciferase reporter gene

   Tags

   Expression

   Mutation

   -947 AP-1 binding site TGAGTCA deletion

   Promoter

   -962 CCND1 promoter

   Available since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pDZ264 (pKAN tdtomato cyc loxP KAN loxP)

   Plasmid

   #35193

   Depositor

   Robert Singer

   Article

   Larson et al Science. 2011 Apr 22;332(6028):475-8.

   Insert

   tdtomato cyc loxP KAN loxP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLINK cyclin T1 (1-280) myc (all 200) (P#575)

    Plasmid

    #14610

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-280) (all 200) (CCNT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    C198A, C200A, H202A, C205A. Active in 3T3 cells.

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y

    Plasmid

    #226349

    Purpose

    Expression of Titin V15P substrate with GS tail

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V15P

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. -962 human cyclin D1 promoter CREB site mutant pGL3Basic

    Plasmid

    #32732

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 Promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -51 CREB site TAACGTCA deletion

    Promoter

    -962 CCND1 Promoter

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

13. -962 human cyclin D1 promoter TCF (0)-(4) sites mutant pGL3Basic

    Plasmid

    #32737

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -539 TCF(0) ATGAAAG deletion; -75 TCF(1) and -68 …

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

14. -962 human cyclin D1 promoter TCF (1) site mutant pGL3Basic

    Plasmid

    #32733

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -75 TCF(1) site CTTTGAT deletion

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

15. -962 human cyclin D1 promoter TCF (1)-(4) sites mutant pGL3Basic

    Plasmid

    #32736

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 Promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

16. -962 human cyclin D1 promoter EtsA site mutant pGL3Basic

    Plasmid

    #32729

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase; Luciferase reporter gene

    Tags

    Expression

    Mutation

    -822 EtsA site AGGAA deletion

    Promoter

    -962 CCND1 promoter

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

17. -962 human cyclin D1 promoter EtsB site mutant pGL3Basic

    Plasmid

    #32730

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -778 EtsB binding site AGGAA deletion

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

18. -962 human cyclin D1 promoter EtsC site mutant pGL3Basic

    Plasmid

    #32731

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -424 EtsC site TTCCT deletion

    Promoter

    -962 CCND1 promoter

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

19. -962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic

    Plasmid

    #32734

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -75 TCF(1) and -68 TCF(2) - CTTTGATCTTTGCT deleti…

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

20. -962 human cyclin D1 promoter TCF (1)-(3) sites mutant pGL3Basic

    Plasmid

    #32735

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 Promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only