Skip to main content
Addgene

We narrowed to 2,013 results for: dox

Showing: 321 - 340 of 2013 results
  1. sgFFL_3x_siRNA

    Plasmid
    #59893
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sites
    Depositor
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--has three siRNA-like targets (fully c…
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. KL095 - pTRE3G-Flag-Halo-CTCF_ZF1_mutant-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #232931
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF ZF1 mutant, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.
    Depositor
    Insert
    Flag-Halo-CTCF ZF1 deletion mutant
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Deleted amino acids 264-277
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pRetroX-Tight-Pur-Trim69 isoform E (codon optimized)

    Plasmid
    #199570
    Purpose
    Stable and dox. inducible expression of Trim69 isoform E (codon optimized) after retroviral-mediated gene transfer
    Insert
    Trim69 isoform E (codon optimized) (TRIM69 Human)
    Use
    Retroviral; Allows for puromycin selection
    Tags
    Flag
    Expression
    Mutation
    codon optimized
    Promoter
    Available since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pRetroX-Tight-Pur-Trim69 isoform D (codon optimized)

    Plasmid
    #199569
    Purpose
    Stable and dox. inducible expression of Trim69 isoform D (codon optimized) after retroviral-mediated gene transfer
    Insert
    Trim69 isoform D (codon optimized) (TRIM69 Human)
    Use
    Retroviral; Allows for puromycin selection
    Tags
    Flag
    Expression
    Mutation
    codon optimized
    Promoter
    Available since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pRetroX-Tight-Pur-Trim69 isoform C (codon optimized)

    Plasmid
    #199568
    Purpose
    Stable and dox. inducible expression of Trim69 isoform C (codon optimized) after retroviral-mediated gene transfer
    Insert
    Trim69 isoform C (codon optimized) (TRIM69 Human)
    Use
    Retroviral; Allows for puromycin selection
    Tags
    Flag
    Expression
    Mutation
    codon optimized
    Promoter
    Available since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. PB-TAC-OK+9MS-KRAB-dCas9

    Plasmid
    #120357
    Purpose
    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Insert
    OK+9MS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [1-483]
    Promoter
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. sgFFL_7x_Bulge

    Plasmid
    #59894
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sites
    Depositor
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--has seven bulge targets (fully comple…
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA3-TrxRFP1

    Plasmid
    #98996
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics
    Depositor
    Insert
    human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP
    Use
    Tags
    His-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pNuc-TrxRFP1

    Plasmid
    #98998
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization
    Depositor
    Insert
    TrxRFP1 and nuclear localization sequence
    Use
    Tags
    His-tag and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. pMito-TrxRFP1

    Plasmid
    #98997
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized
    Depositor
    Insert
    TrxRFP1 and mitochondrial localization sequence
    Use
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
  11. TLCV2-RB1

    Plasmid
    #87836
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.
    Depositor
    Insert
    sgRB1 (RB1 Human)
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tight TRE promoter and U6
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 321 - 340 of 2013 results