We narrowed to 11,252 results for: NSI

1. pAAV-shNCLX-1

   Plasmid

   #181870

   Purpose

   Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

   Depositor

   Hilmar Bading

   Article

   Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

   Insert

   solute carrier 8 family member B1 (Slc8b1 Mouse)
   

   Use

   AAV, Adenoviral, and Mouse Targeting

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW-TetO-Tfap2c

   Plasmid

   #128826

   Purpose

   dox-dependent expression of mouse Tfap2c

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

   Insert

   Tfap2c (Tfap2c Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBp-FGFR3c-S249C

   Plasmid

   #45713

   Depositor

   Matthew Meyerson

   Article

   Liao et al Cancer Res. 2013 Jun 20.

   Insert

   FGFR3 IIIc (FGFR3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S249C

   Promoter

   Available since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

4. mcherry Gamma 9 ---pcDNA3.1

   Plasmid

   #64203

   Purpose

   G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

   Depositor

   Narasimhan Gautam

   Article

   Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

   Insert

   G protein Gamma 9 (GNGT2 Human)
   

   Use

   Tags

   6xHis and mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pT2ADW-lox-mCherryNLS-pA-lox-4493NES

   Plasmid

   #138374

   Purpose

   Booster-PKA for the generation of mice

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   lox-mCherry-lox-Booster-PKA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLPC-MYC.Mm.KAT5

   Plasmid

   #184652

   Purpose

   Retroviral expression of mouse Kat5

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   Kat5 (Kat5 Mouse)
   

   Use

   Retroviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST27-GST-ATXN1 FL(84Q)

   Plasmid

   #21754

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 84 Glutamines and no…

   Promoter

   CMV

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

8. Beclin-BATS

   Plasmid

   #30498

   Depositor

   Qing Zhong

   Article

   Fan et al Proc Natl Acad Sci U S A. 2011 Apr 25. (

   Insert

   Beclin, Barkor (BECN1, ATG14 Human)
   

   Use

   Tags

   3xFlag and hrGFP

   Expression

   Mammalian

   Mutation

   Beclin 1 + 413-492 aa of Barkor

   Promoter

   Available since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pGEX6P1-Ubl-sr1(SIRPT1)

   Plasmid

   #75298

   Purpose

   Expresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)

   Depositor

   Kalle Gehring

   Article

   Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

   Insert

   Human Sacsin 1-339
   

   Use

   Tags

   GST-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBp-FGFR2b-K660N

    Plasmid

    #45707

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIb (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K660N

    Promoter

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pYFP-NGAT(A193T,N194Y)

    Plasmid

    #11388

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Changed Alanine 193 to Threonine, and Asparagine …

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSIL-eGFP-shACTN2

    Plasmid

    #52677

    Purpose

    shRNA against α-actinin-2 with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Insert

    ACTN2 (Actn2 Rat)
    

    Use

    RNAi

    Tags

    CMV-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSW725 - pUFO>>

    Plasmid

    #116000

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pUFO:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp6:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Use

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSETC-RdgBbeta-splice variant 1

    Plasmid

    #58276

    Purpose

    bacterial expression of human RdgBbeta splice variant 1

    Depositor

    Shamshad Cockcroft

    Article

    Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

    Insert

    RdgBbeta (PITPNC1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pYPQ239

    Plasmid

    #108859

    Purpose

    FnCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    FnCpf1
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    Cpf1 is rice codon optimized

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21b-LOV-ipaA

    Plasmid

    #40236

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Jα helix region hybridized (AsLOV2 aa 537-546/ipa…

    Promoter

    T7

    Available since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pSRW1JZ

    Plasmid

    #129528

    Purpose

    C. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pair

    Depositor

    Noelle L'Etoile

    Article

    Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

    Insert

    FlincG3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. (p)odr-3::GFP::EGL-4

    Plasmid

    #100897

    Purpose

    GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

    Depositor

    Noelle L'Etoile

    Article

    Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

    Insert

    56-2741: odr-3 promoter
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    odr-3

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mEos3.2trCD4o

    Plasmid

    #203751

    Purpose

    Encodes the transmembrane domain from CD4, including its 2 palmitoylation sites, with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trCD4o (CD4 Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUDE731

    Plasmid

    #103008

    Purpose

    S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    Fncpf1
    

    Use

    Tags

    3HA and NLS

    Expression

    Yeast

    Mutation

    human codon optimized

    Promoter

    TEF1

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

    Plasmid

    #163698

    Purpose

    AAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortex

    Depositor

    Gina Turrigiano

    Article

    Trojanowski et al Neuron. 2021 Feb 17;109(4):663-676.e5. d

    Insert

    CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII fragment

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNCST-AvicFP1

    Plasmid

    #129504

    Purpose

    Expresses AvicFP1 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAav-MCS-PQS2-3xHA

    Plasmid

    #84917

    Purpose

    rAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

    Insert

    LOX-PGK-NEO-LOX
    

    Use

    AAV

    Tags

    PQS2 3xHA

    Expression

    Mutation

    Promoter

    mPGK

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAS_4xG1 PylT FLAG-G1 PylRS

    Plasmid

    #154768

    Purpose

    plasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNCST-AausGFP

    Plasmid

    #129503

    Purpose

    Expresses AausGFP constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pL90-Nat-2xHO

    Plasmid

    #139069

    Purpose

    CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Insert

    Two guide RNAs targeting the HO endonuclease
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TDH3

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLJM5-mDPYD

    Plasmid

    #61614

    Purpose

    The mouse Dpyd was used to rescue the human DPYD hairpin affect on EMT

    Depositor

    David Sabatini

    Article

    Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

    Insert

    dihydropyrimidine dehydrogenase (Dpyd Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDEST27-GST-ATXN1 FL(30Q)

    Plasmid

    #21753

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 32 amino acids (30 Q…

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pNCST-AvicFP2

    Plasmid

    #129505

    Purpose

    Expresses AvicFP2 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNCST-AausFP2

    Plasmid

    #129500

    Purpose

    Expresses AausFP2 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDEST40-2XFL-Src-Y530EEI

    Plasmid

    #140323

    Purpose

    Expresses Flag-tagged human Src-Y530EEI in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Y530EEI

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-MPRA-MluI-SpeI-EcoRI

    Plasmid

    #190196

    Purpose

    Empty vector for inserting MPRA libraries into AAV

    Depositor

    Hyejung Won

    Article

    McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

    Type

    Empty backbone

    Use

    AAV; Massively parallel reporter assay (mpra)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Kif1A(1-489)-DHFR-myc

    Plasmid

    #117833

    Purpose

    Constitutively active Kinesin-3 motor tagged with DHFR and myc

    Depositor

    Thomas Schwarz

    Article

    Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

    Insert

    Kif1A (1-489) (Kif1a Mouse)
    

    Use

    Tags

    DHFR and myc

    Expression

    Mutation

    only aa 1-489 (motor domain)

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. prk5-TMEM192-3xHA PM mutant

    Plasmid

    #149506

    Purpose

    Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Transient expression

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    p.LL23-24AA, p.LL28-29AA

    Promoter

    CMV

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. gRNA-hIRF-1 #12/pSIR

    Plasmid

    #61080

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    gRNA_hIRF1 promoter #12 (IRF1 Human)
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pX330A-Yap1-E1-#1

    Plasmid

    #171517

    Purpose

    deletion of a genomic locus in Yap1 gene

    Depositor

    Hans Schöler

    Article

    Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

    Insert

    Yap1 (Yap1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pX330A-Yap1-E2-#1

    Plasmid

    #171518

    Purpose

    deletion of a genomic locus in Yap1 gene

    Depositor

    Hans Schöler

    Article

    Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

    Insert

    Yap1 (Yap1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHH0103_CRK-1/2

    Plasmid

    #91367

    Purpose

    Protein expression and purification of human SH3 domain construct CRK-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CRK-1/2 (CRK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGL3-Tet1 promoter A

    Plasmid

    #63881

    Purpose

    Luciferase reporter plasmid used to study transcriptional control of murine Tet1

    Depositor

    Kian Peng Koh

    Article

    Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

    Insert

    Tet1 promoter fragment A
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAS_4xMx PylT C41CA_FLAG_Mx PylRS

    Plasmid

    #140013

    Purpose

    Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge