We narrowed to 17,418 results for: She

1. pENTR/hBIG1

   Plasmid

   #226251

   Purpose

   Entry plasmid of hBIG1 for Gateway system

   Depositor

   Hye-Won Shin

   Insert

   BIG1 (ARFGEF1 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR/HA-hBIG2

   Plasmid

   #226252

   Purpose

   Entry plasmid of N-terminally HA-tagged hBIG2 for Gateway system

   Depositor

   Hye-Won Shin

   Insert

   BIG2 (ARFGEF2 Human)
   

   Use

   Entry vector

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti puro HA-VIM

   Plasmid

   #227585

   Purpose

   Lentiviral plasmid expressing human VIM protein

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   VIM (VIM Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)

   Plasmid

   #225432

   Purpose

   Transcriptional unit of BeYDV Rep/RepA (C1:C2)

   Depositor

   Diego Orzaez

   Insert

   pNOS:Rep/RepA:tNOS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN_TT DNM1L-V5

   Plasmid

   #220359

   Purpose

   Gateway entry vector for an inducible V5-tagged DNM1L

   Depositor

   Kevin Janes

   Insert

   DNM1L (DNM1L Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.SLC17A2-flag

   Plasmid

   #224419

   Purpose

   Expresses SLC17A2 in mammalian cells

   Depositor

   Jonathan Long

   Insert

   Solute carrier family 17 (sodium phosphate), member 2 (SLC17A2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CD55-Delta3

   Plasmid

   #220872

   Purpose

   Expresses CD55 variant in mammalian cells

   Depositor

   Ferenc Scheeren

   Insert

   CD55 Delta-3
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta-3, short consensus repeat (SCR) domains 1, …

   Promoter

   CMV

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CD55-Delta4

   Plasmid

   #220873

   Purpose

   Expresses CD55 variant in mammalian cells

   Depositor

   Ferenc Scheeren

   Insert

   CD55 Delta-4
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta-4, short consensus repeat (SCR) domains 1, …

   Promoter

   CMV

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CD55-Delta1

   Plasmid

   #220870

   Purpose

   Expresses CD55 variant in mammalian cells

   Depositor

   Ferenc Scheeren

   Insert

   CD55 Delta-1
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta-1, the first short consensus repeat (SCR) d…

   Promoter

   CMV

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CD55-Delta2

    Plasmid

    #220871

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-2
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-2, short consensus repeat (SCR) domains 1 a…

    Promoter

    CMV

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. hs-cre::EBD, UAS-dBrainbow attB

    Plasmid

    #220326

    Purpose

    expresses a heat-shock inducible cre::EBD fusion protein and UAS-dBrainbow

    Depositor

    Erdem Bangi

    Inserts

    Cre::EBD (with intron)
    dBrainbow
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS and hs

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDF-B-pC

    Plasmid

    #213495

    Purpose

    Encodes Node B of the three-node Turing circuit

    Depositor

    Mark Isalan

    Inserts

    cinI (cinI )
    lacI (lacI )
    lambda cI (lambdap50 )
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    ASV tag and LVA tag

    Expression

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYES2/NTA/Leu-LegA7Δank

    Plasmid

    #216519

    Purpose

    Expression of LegA7 ΔAnk

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔAnk
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 331-454

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYES2/NTA/Leu-LegA7ΔAnk290

    Plasmid

    #216520

    Purpose

    Expression of LegA7 ΔAnk290

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔAnk290
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 290-454

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYES2/NTA/Leu-LegA7ΔNAnk264

    Plasmid

    #216521

    Purpose

    Expression of LegA7 ΔNAnk264

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔNAnk264
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 264-361

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYES2/NTA/Leu-LegA7ΔNAnk290

    Plasmid

    #216522

    Purpose

    Expression of LegA7 ΔNAnk290

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔNAnk290
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 290-361

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-RPL3L-TwStrep

    Plasmid

    #208649

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949

    Depositor

    Quanfu Ma

    Insert

    human RPL3L (ENST00000268661) (RPL3L Human)
    

    Use

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    contain rs753338989, rs746044959, rs752134949, pr…

    Promoter

    T7

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEB018

    Plasmid

    #215568

    Purpose

    Fuses insert of interest to an HA-tag, stop codon and S. commune Hom2 terminator, with flanks directing the construct to S. commune targeted integration locus

    Depositor

    Robin Ohm

    Insert

    3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
    

    Use

    Fungal homologous recombination plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-CAG-K-red-SPOTIT(R271F)

    Plasmid

    #191494

    Purpose

    Red fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

    Depositor

    Wenjing Wang

    Insert

    K-red-SPOTIT(R271F)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    R271F in OR

    Promoter

    CAG

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-CAG-M-red-SPOTIT2(R279F)

    Plasmid

    #191495

    Purpose

    Red fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

    Depositor

    Wenjing Wang

    Insert

    M-red-SPOTIT2(R279F)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    R279F in OR

    Promoter

    CAG

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET50_G495R MDA5

    Plasmid

    #216803

    Purpose

    Expresses a single point mutant of melanoma differentiation-associated protein 5

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 (IFIH1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G495R

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTRIPZ-NUDT17-C-HA-Puro

    Plasmid

    #195182

    Purpose

    Lentiviral vector for DOX-inducible NUDT17 gene expression with C-terminal HA tag. Gateway expression system. Selection by puromycin.

    Depositor

    Stephanie Roessler

    Insert

    NUDT17 (NUDT17 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter and minimal CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT7CFE1_polio_3ABC_C147R

    Plasmid

    #212985

    Purpose

    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Poliovirus Type I Mahoney 3ABC C147R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Expression

    Mutation

    cysteine 147 in 3C changed to arginine

    Promoter

    T7

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT7CFE1_EVA71_VP12A_C110A

    Plasmid

    #212976

    Purpose

    Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Enterovirus A71 4643 VP12A C110A
    

    Use

    Mammalian cell free protein expression

    Tags

    HA tag

    Expression

    Mutation

    changed cysteine 110 from 2A to alanine

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT7CFE1_polio_VP12A_C109R

    Plasmid

    #212987

    Purpose

    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Poliovirus Type I Mahoney VP12A C109R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Expression

    Mutation

    Cysteine 109 from 2A mutated to arginine

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCHA_SBV_NSs

    Plasmid

    #208938

    Purpose

    Expresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells

    Depositor

    Gerald Barry

    Insert

    NSs
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted 47 bp at 5' end and 510 bp from 3&#x…

    Promoter

    CMV

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28-Flag-RBM24-95~236

    Plasmid

    #208634

    Purpose

    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    only aa 95~236, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid

    #174901

    Purpose

    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAGW-Fray

    Plasmid

    #203979

    Purpose

    GFP-Fray under actin promoter for cell culture

    Depositor

    Andreas Jenny

    Insert

    Frayed (fray Fly)
    

    Use

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAMW-WnkAAxV

    Plasmid

    #203980

    Purpose

    Myc6 Wnk under actin promoter for cell culture

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Use

    Tags

    6xMyc

    Expression

    Insect

    Mutation

    RFxV mutated to AAxV

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. lenti-Cas9-Exo-Blast-BFP

    Plasmid

    #196721

    Purpose

    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-Exo1-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGB-06-07-Vps34

    Plasmid

    #187989

    Purpose

    Plasmid for the protein expression of Vps34 in a bacterial and insect expression system. Internal_Reference: SMC1323

    Depositor

    Sascha Martens

    Insert

    Vps34 (PIK3C3 Human)
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid

    #185061

    Purpose

    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445

    Depositor

    Bobby Koeleman

    Insert

    U6:pegRNA:scaffold:PBS+RT template
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p2151 pHAGE-hSPC-TFEB-TagBFP-W

    Plasmid

    #188722

    Purpose

    Lentiviral vector allowing for SFTPC-driven expression of TFEB:tagBFP fusion protein

    Depositor

    Darrell Kotton

    Insert

    TFEB (TFEB Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mutation

    Promoter

    hSP-C

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCW57.1-GFP_mmu-miR-292b-3p_sponge

    Plasmid

    #132622

    Purpose

    Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

    Depositor

    Constance Ciaudo

    Insert

    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mT2A-TagRFP-T-linker-dre-PBD*-FNF

    Plasmid

    #182342

    Purpose

    Recombineering cassette plasmid for T2A-TagRFP-T-linker-dre-PBD*-FNF

    Depositor

    Mario Capecchi

    Insert

    T2A-TagRFP-T-linker-dre-PBD*-FNF
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CNN3A-c007

    Plasmid

    #175457

    Purpose

    Protein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.

    Depositor

    Opher Gileadi

    Insert

    CNN3 (CNN3 Human)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLV V5-Eva1b

    Plasmid

    #175158

    Purpose

    Lentiviral expression of V5-tagged mouse Eva1b

    Depositor

    Larry Gerace

    Insert

    Eva1b (Eva1b Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLV Cenpv-V5

    Plasmid

    #175125

    Purpose

    Lentiviral expression of mouse Cenpv-V5

    Depositor

    Larry Gerace

    Insert

    Cenpv (Cenpv Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pX330.orup

    Plasmid

    #110404

    Purpose

    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge