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We narrowed to 15,949 results for: LEA

Showing: 8451 - 8500 of 15949 results

  2. pExp-NHis

    Plasmid
    #112558
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112558-plasmid-map-sequence-id-217235

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  3. pCAGG-NFIC2

    Plasmid
    #112701
    Purpose
    Expression of NFIC2 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I C2 (Nfic Mouse)
    Use
    Tags
    GFP and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112701-plasmid-map-sequence-id-212389

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  4. pET28-MHL

    Plasmid
    #26096
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26096-plasmid-map-sequence-id-326568

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  5. pExp-NusA

    Plasmid
    #112571
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112571-plasmid-map-sequence-id-220666

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  6. pCAGG-NFIB2

    Plasmid
    #112700
    Purpose
    Expression of NFIB2 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I B2 (Nfib Mouse)
    Use
    Tags
    GFP and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112700-plasmid-map-sequence-id-212390

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  7. pExp-Sumo

    Plasmid
    #112574
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112574-plasmid-map-sequence-id-217243

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  9. pNIC28-Bsa4-TF

    Plasmid
    #61689
    Purpose
    E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences inserted
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    HIS and Trigger Factor
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pOpen-RNAse H

    Plasmid
    #165575
    Purpose
    Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    RNAse H
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165575-plasmid-map-sequence-id-340983

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  11. pFBOH-LIC

    Plasmid
    #26099
    Purpose
    SGC Empty baculovirus expression/transfer vector
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    6x His and Thrombin protease cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Available Since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26099-plasmid-map-sequence-id-12255

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  12. Nesprin3-C-18

    Plasmid
    #58194
    Purpose
    Localization: Nuclear Membrane , Excitation: N/A, Emission: N/A
    Depositor
    Michael Davidson
    Insert
    Nesprin3 (SYNE3 Human)
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. mCherry-Nucleus-7

    Plasmid
    #55110
    Purpose
    Localization: Nucleus, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    mCherry-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pOpen-SfiIR (cDNA)

    Plasmid
    #165528
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    SfiI (used to create cDNA libraries)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165528-plasmid-map-sequence-id-339067

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  15. pFBOH-MHL

    Plasmid
    #62304
    Purpose
    It is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    6xHis and TEV cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62304-plasmid-map-sequence-id-102204

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  17. mEmerald-Nup35-C-18

    Plasmid
    #54207
    Purpose
    Localization: Nuclear Pore, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nup35 (NUP35 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. mEmerald-Nup50-C-10

    Plasmid
    #54209
    Purpose
    Localization: Nuclear Pore, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nup50 (NUP50 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. NET29b.Mm pEGFP-N2 (1327)

    Plasmid
    #62046
    Purpose
    mammalian expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET29b (Tmem120b Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62046-plasmid-map-sequence-id-101295

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  20. C79m

    Plasmid
    #120991
    Purpose
    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdA antitoxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120991-plasmid-map-sequence-id-236074

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  21. DsRed2-Nucleus-4

    Plasmid
    #55839
    Purpose
    Localization: Nucleus, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    DsRed2-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. NET29A.Mm pEGFP-N2 (1326)

    Plasmid
    #62045
    Purpose
    mammalian expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET29A (Tmem120a Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62045-plasmid-map-sequence-id-101294

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  23. mEmerald-Nup50-N-10

    Plasmid
    #54210
    Purpose
    Localization: Nuclear Pore, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nup50 (NUP50 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. mEmerald-Nup35-N-14

    Plasmid
    #54208
    Purpose
    Localization: Nuclear Pore, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nup35 (NUP35 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. mPlum-Nucleus-7

    Plasmid
    #55991
    Purpose
    Localization: Nucleus, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    mPlum-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. mRuby-Nucleus-7

    Plasmid
    #55875
    Purpose
    Localization: Nucleus, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    mRuby-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. pETcon(I-LtrI)

    Plasmid
    #41523
    Depositor
    Andrew Scharenberg
    Insert
    I-LtrI
    Use
    Tags
    3x(G4S) Linker, G3S Linker, HA Tag, and Myc Tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available Since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    41523-plasmid-map-sequence-id-59600

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  28. Dendra2-LaminA-C-18

    Plasmid
    #57727
    Purpose
    Localization: Nuclear Envelope, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    LaminA
    Use
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. Dronpa-Nucleus-9

    Plasmid
    #57290
    Purpose
    Localization: Nucleus, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    Dronpa-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57290-plasmid-map-sequence-id-183119

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  30. C27m

    Plasmid
    #120965
    Purpose
    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    tetR (no deg tags)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120965-plasmid-map-sequence-id-236387

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  31. pCMV-NFIB2ERt2

    Plasmid
    #112703
    Purpose
    Expression of NFIB2-ERT2 fusion protein
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I B2 (Nfib Mouse)
    Use
    Tags
    ERT2 and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112703-plasmid-map-sequence-id-214845

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  32. C98m

    Plasmid
    #121002
    Purpose
    MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Gp2 toxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121002-plasmid-map-sequence-id-236135

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  33. C51m

    Plasmid
    #120975
    Purpose
    MoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfYFP (has BbsI site)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120975-plasmid-map-sequence-id-236029

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  34. C33m

    Plasmid
    #120968
    Purpose
    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AiiA degradase (Bennett, no ssra tag)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120968-plasmid-map-sequence-id-238044

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  35. HcRed1-Nucleus-7

    Plasmid
    #55843
    Purpose
    Localization: Nucleus, Excitation: 588, Emission: 618
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    HcRed1-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. NET29A.Hs pRRLSIN (1742)

    Plasmid
    #62052
    Purpose
    lentiviral expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET29 (TMEM120A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available Since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62052-plasmid-map-sequence-id-101296

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  37. pQdCas12a.empty

    Plasmid
    #236036
    Purpose
    The plasmid pQdCas12a.empty expresses the dCas12a endonuclease.
    Depositor
    Radhakrishnan Mahadevan
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236036-plasmid-map-sequence-id-474357

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  38. pTYB1-T4denV

    Plasmid
    #228216
    Purpose
    Expression in BL21(DE3) of T4 endonuclease V N-glycosylase UV repair enzyme
    Depositor
    Stephen Benkovic
    Insert
    denV
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228216-plasmid-map-sequence-id-455996

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  39. U17m

    Plasmid
    #121023
    Purpose
    MoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Bujard RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121023-plasmid-map-sequence-id-245898

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  40. U19m

    Plasmid
    #121024
    Purpose
    MoClo golden gate assembly BC part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121024-plasmid-map-sequence-id-238066

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  41. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

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  42. C91m

    Plasmid
    #120999
    Purpose
    MoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfCFP (Chen, Bennett)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120999-plasmid-map-sequence-id-236129

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  43. C82m

    Plasmid
    #120993
    Purpose
    MoClo golden gate assembly CD part for RpaI (synthase for Rpa-detected quorum sensing molecule 4-coumaroyl-homoserine lactone). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RpaI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120993-plasmid-map-sequence-id-236098

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  44. C38m

    Plasmid
    #120971
    Purpose
    MoClo golden gate assembly CD for sigma38/RpoS (RNA polymerase active at transition from exponential phase to stationary phase). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma 38 (RpoS)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120971-plasmid-map-sequence-id-236023

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  45. C21m

    Plasmid
    #120962
    Purpose
    MoClo golden gate assembly CD part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120962-plasmid-map-sequence-id-238043

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  46. p3m

    Plasmid
    #120935
    Purpose
    MoClo golden gate assembly AB part for J23150 (strength ~400x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23150
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120935-plasmid-map-sequence-id-235944

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  47. p4m

    Plasmid
    #120936
    Purpose
    MoClo golden gate assembly AB part for J23151 (strength ~900x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23151
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120936-plasmid-map-sequence-id-235945

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  48. pJK681

    Plasmid
    #72271
    Purpose
    Produces Synechocystis PCC6803 orf sll1489 (synthetic gene)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein SYNGTS_3005
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72271-plasmid-map-sequence-id-131742

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  49. pJK588

    Plasmid
    #72270
    Purpose
    Synthetic gene corresponding to Synechocystis PCC6803 orf sll1489
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein SYNGTS_3005
    Use
    Gene cloning
    Tags
    His6 and TEV cleavage site
    Expression
    Mutation
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72270-plasmid-map-sequence-id-131740

    Enlarge

  50. pAAV_BiPVe3_GCaMP6f (AAV PHP.eB)

    Viral Prep
    #213943-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV_BiPVe3_GCaMP6f (#213943). In addition to the viral particles, you will also receive purified pAAV_BiPVe3_GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the PV+ basket cell-targeting enhancer E3. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Promoter
    Tags
    None
    Available Since
    Sept. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 8451 - 8500 of 15949 results