We narrowed to 5,634 results for: chia

1. pTol2CG2-ubi:QFGal4-SV40pA

   Plasmid

   #155119

   Purpose

   Tol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   ubi:QFGal4 (ubb Zebrafish, Budding Yeast, Neurospora crassa)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   ubiquitin B

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHOT-P2Y2R

   Plasmid

   #159108

   Purpose

   entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson

   Depositor

   Fabio Mammano

   Article

   Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc

   Insert

   P2Y2R
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   UBC

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

   Plasmid

   #100596

   Purpose

   piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Inserts

   CRISPR Cas9 fused with human Glucocorticoid Receptor
   mCherry
   rtTA-M2
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human codon usage

   Promoter

   Dox-inducible TetO promoter and Human EEF1A1 prom…

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLS-4LNDDE488Q-pCDNA3.1(+)

   Plasmid

   #172314

   Purpose

   Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)

   Depositor

   Joshua Rosenthal

   Article

   Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

   Insert

   Lambda N peptide - human ADAR2 Deaminase Domain E488Q
   

   Use

   Tags

   6xHis, Flag, and Nuclear Localization Signal of S…

   Expression

   Mammalian

   Mutation

   changed the Glutamic acid 488 in the Deaminase Do…

   Promoter

   CMV

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pScalps_Puro_mTet2 catalytic domain HxD

   Plasmid

   #79611

   Purpose

   Expression of catalytically inactive mouse Tet2

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Tet2 (Tet2 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mutation

   Mutant Tet2 with H1302Y, D1304A substitutions in …

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pelB-MBP-P66-ss

   Plasmid

   #137045

   Purpose

   E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66949.1 (p66 Borrelia burgdorferi B31)
   

   Use

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature P66: lacks the P66 signal peptide

   Promoter

   T7lac

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSPL3-hRPH3A_c.1853Gmut

   Plasmid

   #190479

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.1853A>G

   Promoter

   SV40

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pelB-MBP-P13-ss

   Plasmid

   #137065

   Purpose

   E. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
   

   Use

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature P13: lacks the P13 signal peptide

   Promoter

   T7lac

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-Twin-Strep-TEV-DGKζ

   Plasmid

   #202531

   Purpose

   Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   DGKZ (DGKZ Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and chicken β-actin promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-PGK-Chst3

    Plasmid

    #193024

    Purpose

    AAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoter

    Depositor

    Bart Nieuwenhuis

    Article

    Yang et al Mol Psychiatry. 2021 Jul 16. pii: 10.103

    Insert

    Chst3 (Chst3 Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pdCas9-M-G6

    Plasmid

    #73437

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor G6 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    March 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCas9-M-3A2

    Plasmid

    #73435

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 3A2 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    March 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDest-1.7kbfabp6-eGFP-pA-CrymCherry

    Plasmid

    #159088

    Purpose

    LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts

    Depositor

    John Rawls

    Article

    Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

    Inserts

    1.7kb fabp6 promoter (fabp6 Zebrafish)
    eGFP
    polyA
    

    Use

    Fluorescent reporter

    Tags

    Expression

    Mutation

    Promoter

    1.7kb fabp6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pdCas9-M-4A6

    Plasmid

    #73434

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 4A6 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pelB-MBP-RevA-ss

    Plasmid

    #137064

    Purpose

    E. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAF07416.1 (revA Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    lacks the RevA signal peptide and 5 residues (CKA…

    Promoter

    T7lac

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Syn-SomArchon-mTagBFP2-p2A-CoChR-Kv2.1

    Plasmid

    #206006

    Purpose

    Expression of SomArchon-mTagBFP2 and CoChR under Syn promoter

    Depositor

    Xue Han

    Article

    Lowet et al Cell Rep. 2023 Aug 29;42(8):112906. doi:

    Insert

    SomArchon-mTagBFP2-P2A-CoChR-Kv2.1
    

    Use

    AAV

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pelB-MBP-HtrA-ss

    Plasmid

    #137066

    Purpose

    E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature HtrA: lacks the HtrA signal peptide

    Promoter

    T7lac

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pelB-MBP-DipA-ss

    Plasmid

    #137068

    Purpose

    E. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature DipA: lacks the DipA signal peptide

    Promoter

    T7lac

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTO_Snf7_LAP

    Plasmid

    #101851

    Purpose

    Tet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

    Depositor

    Daniel Gerlich

    Article

    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

    Insert

    SNF7 (SNF7 Budding Yeast)
    

    Use

    Gateway cloning, flp-frt recombination, tet-induc…

    Tags

    Localization and Affinity Purification (LAP) tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-DDX11-Flag-KAE

    Plasmid

    #120728

    Purpose

    Expression of a Flag-tagged version of DDX11 KAE mutant in mammalian cells

    Depositor

    Francesca Pisani

    Article

    Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

    Insert

    DDX11 KAE mutant (DDX11 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Substitution of E201 and Y202 with K and A

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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