We narrowed to 5,724 results for: chia

1. Lenti_CRISPRi_sgAR3

   Plasmid

   #167002

   Purpose

   Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   AR Promoter sgRNA 3 (AR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. scAAV-TTR-mFgf15

   Plasmid

   #190594

   Purpose

   AAV construct containing Fgf15 cds under control of TTR promoter

   Depositor

   Roel Nusse

   Article

   Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

   Insert

   Fgf15 (Fgf15 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ttr

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM6-3F2-mCherry

   Plasmid

   #73418

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 3F2 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P3F2 (orthogonal T7-lac variant)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-pro-siRNA-V2-LMNA

   Plasmid

   #111088

   Purpose

   pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human Lamin A/C gene.

   Depositor

   Linfeng HUANG

   Article

   Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

   Insert

   Lamin A/C (LMNA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPICZ-pre-Ost1-alphaf(I)-Btl2

   Plasmid

   #117667

   Purpose

   Study secretion efficiency of Btl2

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-Ost1-alphaf(I)-Btl2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the lipase Btl2 together wit…

   Promoter

   pAOX1

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500

   Plasmid

   #92225

   Purpose

   Expression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   deGFP Y35TAG
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Residue Y35 mutated into TAG (Amber) stop codon f…

   Promoter

   p70a

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM6-3A2-mCherry

   Plasmid

   #73425

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 3A2 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P3A2 (orthogonal T7-lac variant)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM6-5F5-mCherry

   Plasmid

   #73422

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 5F5 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P5F5 (orthogonal T7-lac variant)

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV- GfaABC1D::Cas9-HA-sgRNA-Non-Targeting

   Plasmid

   #241026

   Purpose

   GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of a non-targeting sgRNA; used as control for SaCas9 based knock-outs in murine astrocytes

   Depositor

   Mark Verheijen

   Article

   Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

   Insert

   U6 driven empty sgRNA
   

   Use

   AAV and CRISPR

   Tags

   SaCas9 + 3X HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   GfaABC1D

   Available since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-WzxE-TEV-His10

    Plasmid

    #226781

    Purpose

    Expression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coli

    Depositor

    James Naismith

    Article

    Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

    Insert

    wzxE
    

    Use

    Tags

    TEV cleavable 10xHistidine tag

    Expression

    Bacterial

    Mutation

    valine insertion in position 2 due to the cloning…

    Promoter

    araBAD

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA5-TEX264-SIM1_mut-cGFP

    Plasmid

    #220212

    Purpose

    Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

    Promoter

    CMV/TetO

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

    Plasmid

    #207344

    Purpose

    Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Δ37, Δ115-133, Δ204-217

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE-80L 2xHis-LRRK2 Armadillo E240R

    Plasmid

    #207345

    Purpose

    Bacterial expression of LRRK2 Armadillo

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    LRRK2 Armadillo (LRRK2 Human)
    

    Use

    Tags

    2xHis

    Expression

    Bacterial

    Mutation

    E240R; Armadillo domain only (aa 1-552)

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE-80L 2xHis-LRRK2 Armadillo K439E

    Plasmid

    #207346

    Purpose

    Bacterial expression of LRRK2 Armadillo

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    LRRK2 Armadillo (LRRK2 Human)
    

    Use

    Tags

    2xHis

    Expression

    Bacterial

    Mutation

    K439E; Armadillo domain only (aa 1-552)

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQE-80L 2xHis-LRRK2 Armadillo F283A

    Plasmid

    #207348

    Purpose

    Bacterial expression of LRRK2 Armadillo

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    LRRK2 Armadillo (LRRK2 Human)
    

    Use

    Tags

    2xHis

    Expression

    Bacterial

    Mutation

    F283A; Armadillo domain only (aa 1-552)

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV Flag-LRRK2 K17A/K18A/R1441G

    Plasmid

    #186013

    Purpose

    Flag-LRRK2 K17A/K18A/R1441G

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    Flag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor's comments below.

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-guide-puro mBltp1 (KIAA1109) - 1

    Plasmid

    #198489

    Purpose

    lentiviral stable expression of mBltp1 (KIAA1109) gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mBltp1 (4932438A13Rik Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-guide-puro mBltp1 (KIAA1109) - 2

    Plasmid

    #198490

    Purpose

    lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mBltp1 (4932438A13Rik Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-guide-puro mAtp6v1a-1

    Plasmid

    #198477

    Purpose

    lentiviral stable expression of mAtp6v1a gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mouse ATPv1a (Atp6v1a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-guide-puro mAtp6v1a-2

    Plasmid

    #198478

    Purpose

    lentiviral stable expression of mAtp6v1a gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mouse ATPv1a (Atp6v1a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge