We narrowed to 5,844 results for: pCas

1. px459 VRER

   Plasmid

   #101716

   Purpose

   sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E and T1337R)

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330A_D10A-1x7

   Plasmid

   #58777

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. sABE v3.22 Lenti_2

   Plasmid

   #209556

   Purpose

   pU6-SpCas9_sgRNA-pCMV-bpSV40_NLS-TadA-8e (2-76)-2xFRB

   Depositor

   Xue Gao

   Article

   Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

   Insert

   SpCas9 sgRNA, TadA-8e (2-76)-2xFRB
   

   Expression

   Mammalian

   Promoter

   pU6, pCMV

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-sgRNA_Dgcr8_1

   Plasmid

   #73525

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pX330A_D10A-1x4

   Plasmid

   #58774

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330A_D10A-1x6

   Plasmid

   #58776

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFB-Flag-MyoD

   Plasmid

   #25994

   Depositor

   Jeffrey Dilworth

   Article

   Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

   Insert

   MyoD (Myod1 Mouse)
   

   Use

   Baculovirus transfer vector

   Tags

   Flag

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pX330A_D10A-1x3

   Plasmid

   #58773

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-sgRNA-com-BoxB-vector

   Plasmid

   #132556

   Purpose

   Expresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.

   Depositor

   Baisong Lu

   Inserts

   dCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
   Lambda N22-KRAB
   sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
   

   Expression

   Mammalian

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-sgRNA_Dgcr8_3

    Plasmid

    #73527

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    DGCR8
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pX330-sgRNA_Dgcr8_4

    Plasmid

    #73528

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    DGCR8
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pX330-sgRNA_Dgcr8_2

    Plasmid

    #73526

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    DGCR8
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pIVT-ABE-DEN2

    Plasmid

    #238020

    Purpose

    for DNA-free adenine base editing in rice and wheat or other plants

    Depositor

    Caixia Gao

    Article

    Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

    Insert

    TadA8e-nSpCas9(D10A)
    

    Expression

    Plant

    Mutation

    D10A for SpCas9

    Promoter

    T7

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIVT-ABE-TMV

    Plasmid

    #238021

    Purpose

    for DNA-free adenine base editing in rice and wheat or other plants

    Depositor

    Caixia Gao

    Article

    Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

    Insert

    TadA8e-nSpCas9(D10A)
    

    Expression

    Plant

    Mutation

    D10A for SpCas9

    Promoter

    T7

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIVT-A3A-DEN2

    Plasmid

    #238019

    Purpose

    for DNA-free cytosine base editing in rice and wheat or other plants

    Depositor

    Caixia Gao

    Article

    Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

    Insert

    A3A-nSpCas9(D10A)-UGI
    

    Expression

    Plant

    Mutation

    D10A for SpCas9

    Promoter

    T7

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQAT06

    Plasmid

    #223378

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-ZmUbi-gRNA scaffold-tRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGG-PIP34

    Plasmid

    #196772

    Purpose

    Contains HPCA1 promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    HP-INDUCED Ca2+ INCREASES 1 (AT5G49760 Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Available Since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p8270 LentiCRISPR v2 hygro sgSIGIRR-3

    Plasmid

    #193979

    Purpose

    Expression of spCas9 and sgRNA targeting SIGIRR

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
    

    Use

    Lentiviral

    Available Since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGGC-LexACasPea

    Plasmid

    #210539

    Purpose

    Cloning estradiol-inducible expression cassette containi LexA XVE binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate module C

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    LexA::SpCas9::PEA3AT
    

    Use

    Synthetic Biology

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDL28_Cas9-His Δcys E532C/E1207C

    Plasmid

    #206289

    Purpose

    Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, E532C, C574S, E1207C

    Promoter

    T7

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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