We narrowed to 72,365 results for: LAS

1. pAAV-G12-hFGF1

   Plasmid

   #219554

   Purpose

   hFGF1 expression in mammalian cells. pAAV vector. Reporter plasmid.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

   Insert

   hFGF1 (FGF1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   12xUAS with TATA-box minimal promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNS49 (enAsCas12a-KRAB piggyBac)

   Plasmid

   #217335

   Purpose

   piggyBac expresion of enAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   enAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   E174R/S542R/K548R

   Promoter

   CAG

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a

   Plasmid

   #213401

   Purpose

   Plant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursor

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:

   Insert

   Arabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pROF018

   Plasmid

   #155325

   Purpose

   Constitutive expression of KRAB-EL222 in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-NLS-KRAB-EL222-Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pROF050

   Plasmid

   #155327

   Purpose

   Constitutive expression of BRD-EL222 in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-BRD-NLS-EL222-Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1-zeo G226A E321K ORF29

   Plasmid

   #200026

   Purpose

   Lentiviral vector for expression of KSHV ORF29 with G226A and E321K mutation

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   ORF29
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glycine 226 changed to alanine, glutamic acid 321…

   Promoter

   CMV

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458_miR-290-295KO-gRNA3

   Plasmid

   #172711

   Purpose

   Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

   Depositor

   Constance Ciaudo

   Article

   Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

   Insert

   miR-290-295
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFAP4_BHLH_5-1

   Plasmid

   #185054

   Purpose

   Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

   Depositor

   Constance Ciaudo

   Article

   Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

   Insert

   TFAP4_5_1_gRNA (Tcfap4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFAP4_BHLH_3-2

   Plasmid

   #185055

   Purpose

   Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_5-1 or TFAP4_BHLH_5-2

   Depositor

   Constance Ciaudo

   Article

   Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

   Insert

   TFAP4_3_2_gRNA (Tcfap4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFAP4_BHLH_5-2

    Plasmid

    #185056

    Purpose

    Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    TFAP4_5_2_gRNA (Tcfap4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. KBB438

    Plasmid

    #185092

    Purpose

    GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Rfa1 with amino acids 338-343 deleted

    Promoter

    GAL1

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. KBB372

    Plasmid

    #185084

    Purpose

    GAL::Rfa1[330-621]-GFP under control of GAL1 promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Rfa1 amino acids 330-621

    Promoter

    GAL1

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. tcTRP24sub8_SH2sub6

    Plasmid

    #175799

    Purpose

    de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing 2 copies of human Nck2 SH2

    Depositor

    Barry Stoddard

    Article

    Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

    Insert

    tcTRP24sub8_SH2sub6
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r

    Plasmid

    #162950

    Purpose

    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed protein

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET_3H1L_02.395

    Plasmid

    #155198

    Purpose

    Expresses 3H1L_02.395 in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    3H1L_02.395
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. KHLH31 pEGFP-N2 (1089)

    Plasmid

    #62032

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    KHLH31
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pBS cSugarCrisp (CT#708)

    Plasmid

    #13956

    Depositor

    Cliff Tabin

    Article

    Smith et al Mech Dev. 2001 Apr . 102(1-2):223-6.

    Insert

    SugarCrisp (PI15 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pBS hSugarCrisp (CT#592)

    Plasmid

    #13966

    Depositor

    Cliff Tabin

    Article

    Smith et al Mech Dev. 2001 Apr . 102(1-2):223-6.

    Insert

    SugarCrisp (PI15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pAV10

    Plasmid

    #63213

    Purpose

    pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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20. EcNGRfEfC-R10S1B9

    Bacterial Strain

    #48895

    Purpose

    diversity was introduced via homologous recombination to 40 sites to improve fatty acid production. Sequencing of the strains at these sites is ongoing.

    Depositor

    George Church

    Bacterial Resistance

    Spectinomycin

    Available since

    Dec. 3, 2014

    Availability

    Academic Institutions and Nonprofits only