We narrowed to 11,554 results for: Vars;

1. GST_LIM1224

   Plasmid

   #182041

   Purpose

   For expression of human paxillin LIM domains 1, 2, 2 and 4 with N-terminal GST fusion protein in E. coli.

   Depositor

   Vesa Hytönen

   Article

   Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

   Insert

   LIM1224 (PXN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   LIM domains 1, 2, 2 and 4

   Promoter

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST_LIM1334

   Plasmid

   #182040

   Purpose

   For expression of human paxillin LIM domains 1, 3, 3 and 4 with N-terminal GST fusion protein in E. coli.

   Depositor

   Vesa Hytönen

   Article

   Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

   Insert

   LIM1334 (PXN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   LIM domains 1, 3, 3 and 4

   Promoter

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pML104-natR412

   Plasmid

   #162042

   Purpose

   Expresses Cas9 and contains natR412 guide which targets the natR gene

   Depositor

   Jolanda Van Leeuwen

   Article

   Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

   Insert

   natR412 guide
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pML107-natR854

   Plasmid

   #162043

   Purpose

   Expresses Cas9 and contains natR854 guide which targets the natR gene

   Depositor

   Jolanda Van Leeuwen

   Article

   Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

   Insert

   natR854 guide
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lmajor_gp63_10_0460_P460G_D463N_S465A

   Plasmid

   #171645

   Purpose

   Expression of L. major glcyoprotein-63 (P460G_D463N_S465A) with substrate binding site mutations from chromosome 10 in mammalian cells

   Depositor

   Dennis Ko

   Article

   Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

   Insert

   LmjF.10.0460 P460G D463N S465A
   

   Use

   Tags

   Myc-His

   Expression

   Mammalian

   Mutation

   P460G; D463N; S465A

   Promoter

   Available Since

   Dec. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJH191

   Plasmid

   #172149

   Purpose

   Accessory Plasmid for PACE of pylBCD variants

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Ppsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gIII(P29*, Y184*); [strong promoter eG, strong RB…

   Promoter

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJH190

   Plasmid

   #172148

   Purpose

   Accessory Plasmid for PACE of pylBCD variants

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gIII(P29*); [strong promoter eG, strong RBS SD8] …

   Promoter

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Negative feedback (NF) plasmid

   Plasmid

   #169747

   Purpose

   Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-tetO2

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMS11: pTns(AvCAST)_ΔTniQ,ΔTnsD

   Plasmid

   #168144

   Purpose

   Inducible expression of AvCAST TnsA, TnsB and TnsC proteins.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   AvCAST Tns proteins (TnsA, TnsB and TnsC)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMS9: pTns(AvCAST)_ΔTniQ

    Plasmid

    #168142

    Purpose

    Inducible expression of AvCAST TnsA, TnsB, TnsC and TnsD proteins.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST Tns proteins (TnsA, TnsB and TnsC)
    AvTnsD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPR V2 sgACO1_3

    Plasmid

    #169893

    Purpose

    Disrupt ACO1

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    sgRNA targeting ACO1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgiRFP1

    Plasmid

    #113972

    Purpose

    Single short guide RNA targeting GATCGAGTTCGAGCCTGCGG in iRFP670 sequence

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    iRFP670 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. sgiRFP2

    Plasmid

    #113973

    Purpose

    Single short guide RNA targeting GCGCGTTCTTTGGACGCGA in iRFP670 sequence

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    iRFP670 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. sgiRFP3

    Plasmid

    #113974

    Purpose

    Single short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequence

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    iRFP670 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. VanR - F165V

    Plasmid

    #158965

    Purpose

    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.

    Depositor

    Michael Jensen

    Article

    et al Biotechnology Notes, Volume 1, 2020, Pag

    Insert

    VanR - F165V
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Wild type VanR from Caulobacter crescentus with m…

    Promoter

    TEF1

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. VanR - F165R

    Plasmid

    #158966

    Purpose

    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.

    Depositor

    Michael Jensen

    Article

    et al Biotechnology Notes, Volume 1, 2020, Pag

    Insert

    VanR - F165R
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Wild type VanR from Caulobacter crescentus with m…

    Promoter

    TEF1

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFJ2365 - Intron GG1 - 250bp no PATC

    Plasmid

    #159883

    Purpose

    Golden Gate compatible intron with no PATCs for negative controls

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG1 - 250bp no PATC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFJ2345 - Intron GG2 - 250bp no PATC

    Plasmid

    #159884

    Purpose

    Golden Gate compatible intron with no PATCs for negative controls

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG2 - 250bp no PATC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFJ2366 - intron GG3 - 250bp no PATC

    Plasmid

    #159885

    Purpose

    Golden Gate compatible intron with no PATCs for negative controls

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG3 - 250bp no PATC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sg1

    Plasmid

    #113966

    Purpose

    Single short guide RNA targeting GTATAGCATACATTATACG

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge