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pYPQ134-STU-Lb

Plasmid

#138105

Purpose

Golden Gate entry vector for 4th crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ133-STU-Lb

Plasmid

#138102

Purpose

Golden Gate entry vector for 3rd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pGT-S2

Plasmid

#122583

Purpose

MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_A0901

Plasmid

#91025

Purpose

Module A, Promoter: 35S, Gene: TREX2-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TREX2-P2A-AtCas9

Use

CRISPR

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0902

Plasmid

#91026

Purpose

Module A, Promoter: AtUbi10, Gene: TREX2-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TREX2-P2A-AtCas9

Use

CRISPR

Promoter

AtUbi10

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pGGC025

Plasmid

#48830

Purpose

Provides 3xGFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

3xGFP

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Rosa26-twinPE-attB

Plasmid

#207859

Purpose

AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PEmaxDRNaseH-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3 GCN1 R2312A-3xFlag_IRES-iRFP

Plasmid

#198389

Purpose

GCN1 R2312A expresson

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

R2312A mutation in RWDBD (RWD binding domain)

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pD3sE_SC12

Plasmid

#219720

Purpose

DV3 E dimer with fusion loop mutation and increased expression

Depositor

Brian Kuhlman

Article

Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

Insert

DV3 sE SC12

Tags

8xHis and human serum albumin (HSA)

Expression

Mammalian

Mutation

G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pWN_U6-TRAC-miniGag

Plasmid

#228960

Purpose

miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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J23100-mRFP1-331Bb

Plasmid

#78271

Purpose

mRFP1 behind constitutive promoter J23100 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23100-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pKMV-VioD

Plasmid

#65350

Purpose

systhesis of vioD gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioD

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pKMV-VioC

Plasmid

#65349

Purpose

systhesis of vioC gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioC

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pR562

Plasmid

#207886

Purpose

Creation of Level 0 entry clones

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

amilCP

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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OA984

Plasmid

#120363

Purpose

Expresses an single chain antibody 1C19 that targets all four DENV serotypes and expresses a red fluorescent protein reporter

Depositor

Omar Akbari

Article

Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.

Insert

1C19 single chain antibody

Use

Unspecified

Promoter

AAEL010782 carboxypeptidase A (CPA) gene Promoter

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEf1a-hifiDn29-dCas9-P2A-GFP

Plasmid

#247158

Purpose

Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-dCas9-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-ExRai-AktAR2

Plasmid

#184049

Purpose

Nuclear-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Nuc-ExRai-AktAR2

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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hMLKL NB(1-140)-2xFv-Venus

Plasmid

#106076

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL NB(1-140)-2xFv-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Mutation

amino acids 1-140

Promoter

Dox-inducible

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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RBD#3L

Plasmid

#193020

Purpose

hnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

Depositor

Xue Gao

Article

Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

Insert

Cas13a

Tags

hnRNP A1 RRM2

Expression

Bacterial

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJZC583

Plasmid

#62314

Purpose

sgRNA with 2x MS2 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x MS2 binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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