We narrowed to 6,889 results for: GFP expression plasmids

1. Danio alpha Bb-crystallin 1kb promoter/AcGFP

   Plasmid

   #98098

   Purpose

   aBb-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

   Depositor

   Mason Posner

   Article

   Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

   Insert

   Alpha Bb-crystallin promoter 1 kb fragment, Danio rerio (cryabb Zebrafish)
   

   Use

   Gfp expressing

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Danio aBb-crystallin 1 kb fragment (-1074/-1)

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Danio alpha Ba-crystallin 3kb promoter/AcGFP

   Plasmid

   #98097

   Purpose

   aBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

   Depositor

   Mason Posner

   Article

   Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

   Insert

   Alpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)
   

   Use

   Gfp expressing

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Danio alpha Ba-crystallin 3 kb fragment (-3000/-1)

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.T7-SP6-BC(p11-14)-CAG-EGFP-W3SL-T7

   Plasmid

   #231348

   Purpose

   Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7-SP6-BC(p11-14)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GFP-IRES-hSNCA (A30P)

   Plasmid

   #185720

   Purpose

   AAV expression of GFP and human α-Synuclein with A30P mutation from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Changed Ala 30 to Pro

   Promoter

   hSyn

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-GFP-IRES-hSNCA (S129D)

   Plasmid

   #185719

   Purpose

   AAV expression of GFP and human α-Synuclein with S129D mutation from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Changed Ser 129 to Asp

   Promoter

   hSyn

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-GFP-IRES-hSNCA (S129A)

   Plasmid

   #185718

   Purpose

   AAV expression of GFP and human α-Synuclein with S129A mutation from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Changed Ser 129 to Ala

   Promoter

   hSyn

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-EGFP-CAAX

   Plasmid

   #208684

   Purpose

   Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   membrane-localized enhanced green fluorescent protein EGFP-CAAX
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124429

   Purpose

   Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(TEV cleavage seq) T2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. XLone-Puro CCND2-T2A-luciferase-P2A-eGFP

   Plasmid

   #179843

   Purpose

   Tunable and temporal expression control of CCND2, luciferase and nuclear eGFP

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Luciferase and Synthetic Biology

   Tags

   eGFP and luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192789

    Purpose

    aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody minimizer
    

    Use

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192788

    Purpose

    aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody enhancer
    

    Use

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

    Plasmid

    #113041

    Purpose

    AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

    Plasmid

    #113040

    Purpose

    AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

    Plasmid

    #113042

    Purpose

    AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP

    Plasmid

    #196874

    Purpose

    Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynGFP
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre;3xP3-gTc’v-SV40]

    Plasmid

    #124068

    Purpose

    CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony for linearization; (Transformation plasmid; Black eye marker)

    Depositor

    Gregor Bucher

    Article

    He et al Elife. 2019 Oct 18;8. pii: 49065. doi: 1

    Insert

    eb-Tc’bhsp-EGFP-2A-Cre; 3xP3-gTc’v-SV40
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Insect

    Mutation

    Promoter

    bhsp68

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-GFP-pA

    Plasmid

    #192500

    Purpose

    To express DjCas13d compatible gRNA and GFP

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    DjCas13d
    

    Use

    AAV

    Tags

    Expression

    Mutation

    No

    Promoter

    CMV

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-UbC-rtTA-Ngn2:2A:EGFP

    Plasmid

    #127288

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

    Insert

    Ngn2:2A:Ascl1 (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and EGFP linked via 2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid

    #208336

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CAG

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Syn-FLEX-rc [ChrimsonR-GFP]

    Plasmid

    #84480

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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