We narrowed to 18,068 results for: Mpl;

1. CaV1.3e[8a,11,31b,Δ32,42a]

   Plasmid

   #49333

   Purpose

   Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

   Depositor

   Diane Lipscombe

   Article

   Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

   Insert

   CACNA1D (Cacna1d Rat)
   

   Expression

   Mammalian

   Mutation

   Contains exons 8a, 11, 31b and 42a.Deletion of ex…

   Promoter

   CMV

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir

   Plasmid

   #90004

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   FKBP-linker-Myc-NBirA*
   CBirA*-linker-FRB
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. myc-Atg14

   Plasmid

   #86747

   Purpose

   N-terminal myc-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

   Insert

   human Atg14 (ATG14 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA 3.3 HA-hTSPAN12

   Plasmid

   #115784

   Purpose

   Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   TSPAN12 (TSPAN12 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   multiple silent mutations introduce restriction s…

   Promoter

   CMV

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCSN067

   Plasmid

   #101748

   Purpose

   Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-PTBP1CCtoSS

   Plasmid

   #89157

   Purpose

   Recombinant expression of PTBP1 C250S,C251S in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)
   

   Tags

   hexa His

   Expression

   Bacterial

   Mutation

   mutations: Cys 250 to Ser, Cys 251 to Ser

   Promoter

   T7

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LV-LIN28A

   Plasmid

   #117057

   Purpose

   Overexpression of mouse LIN28A

   Depositor

   Michele De Palma

   Article

   Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

   Insert

   LIN28A (Lin28a Mouse)
   

   Use

   Lentiviral

   Promoter

   hPGK promoter

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

   Plasmid

   #84261

   Purpose

   Expresses AND gate ABA/GA-inducible VPR-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9
   PYL1-VPR-p2a-GID1-ABI
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …

   Expression

   Mammalian

   Promoter

   CAG and PGK

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBABE-Flag-Cdk9-D167N-IRES-eGFP

   Plasmid

   #28098

   Depositor

   Andrew Rice

   Article

   Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

   Insert

   Cdk9 (CDK9 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains D167N mutation in Cdk9

   Available Since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir

    Plasmid

    #90009

    Purpose

    Split-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    FKBP-linker-Myc-NBirA*
    FRB-linker-CBirA*
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-CHC17KDP

    Plasmid

    #59799

    Purpose

    Expresses knockdown-proof human CHC17 tagged with GFP.

    Depositor

    Stephen Royle

    Article

    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

    Insert

    CHC17 (CLTC Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Silent mutations in amino acids 61-65 to confer s…

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBABE-Flag-Cdk9-T186A-IRES-eGFP

    Plasmid

    #28097

    Depositor

    Andrew Rice

    Article

    Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains T186A mutation in Cdk9

    Available Since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1 HA-FAM208A

    Plasmid

    #115845

    Purpose

    Encodes codon optimized HA tagged FAM208A

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HA tagged FAM208A (C3orf63 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJZC34

    Plasmid

    #62331

    Purpose

    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2x MS2(wt+f6) binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCIneo-lambdaN-HA-HsEDC4

    Plasmid

    #66597

    Purpose

    Expresses lambdaN-HA-tagged EDC4 in mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    EDC4 (EDC4 Human)
    

    Tags

    lambdaN-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA-mSin1(1.1)-HA

    Plasmid

    #73388

    Purpose

    Expresses mSin-HA

    Depositor

    Jie Chen, Taekjip Ha

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    mSin1 transcript variant protein isoform 1 (MAPKAP1 Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs-IP-EGFP-hAtg13

    Plasmid

    #38191

    Depositor

    Noboru Mizushima

    Article

    Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

    Insert

    autophagy-related genes 13 (ATG13 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

18. Flag-betaPIXa

    Plasmid

    #15234

    Depositor

    Rick Horwitz

    Article

    Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

    Insert

    beta-PIXa (Arhgef7 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3 barr1 flag

    Plasmid

    #14687

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    beta arrestin 1 (Arrb1 Rat)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

20. Flag-ECFP-betaPIXa

    Plasmid

    #15235

    Depositor

    Rick Horwitz

    Article

    Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

    Insert

    beta-PIXa (Arhgef7 Mouse)
    

    Tags

    ECFP and Flag

    Expression

    Mammalian

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only