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pCfB8379

Plasmid

#126913

Purpose

Plasmid containing integration cassette at X-4 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and XdCrtYB

Depositor

Irina Borodina

Publication

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and XdCrtYB

Expression

Yeast

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0641

Plasmid

#129006

Purpose

Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTEF6-7

Plasmid

#124583

Purpose

Reporter plasmid with constitutively expressed mCherry and YFP (eCitrine)

Depositor

Matthias Heinemann

Publication

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

mCherry

Expression

Yeast

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJK148-Pnmt41-GBP-mCherry(N)-leu1+

Plasmid

#89070

Purpose

genomic GBP-mCherry epitope N-terminal tagging at leu1+ locus in S. pombe

Depositor

Quanwen Jin

Publication

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Genomic integration in s. pombe

Tags

GBP-mCherry

Expression

Yeast

Available since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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MCV

Plasmid

#81174

Purpose

MCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 cloning of inserts

Depositor

Serge Pelet

Publication

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDZ585 pKAN 2x-mCherry

Plasmid

#72236

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

Depositor

Daniel Zenklusen

Publication

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

2x-mCherry

Expression

Bacterial

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-FLJ23445

Plasmid

#15429

Depositor

Joan Conaway, Ronald Conaway

Publication

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

FLJ23445 (Thoc7 Mouse)

Tags

FLAG

Expression

Mammalian

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pET41a-MED22

Plasmid

#15432

Depositor

Joan Conaway, Ronald Conaway

Publication

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED22 (MED22 Human)

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pRS406-Padh-OsTIR1(F74G)

Plasmid

#247604

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRS405-Padh-OsTIR1(F74G)

Plasmid

#247603

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCfB8742

Plasmid

#126908

Purpose

Template for PCR amplification of pGPD1-pFBA1 double promoter biobrick for Yarrowia lipolytica

Depositor

Irina Borodina

Publication

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

pGPD1 and pFBA1

Use

Synthetic Biology

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0612

Plasmid

#133505

Purpose

Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0526

Plasmid

#133500

Purpose

Expression of sfGFP targeted to the nucleus with one NLS signals

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(tdh1)-sfGFP-NLSSV40-natMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0785

Plasmid

#133507

Purpose

Expression of mCherry tagged PCNA to monitor DNA replication

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(pcn1)-mCherry-pcn1-terminator(nmt1)-natMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0620

Plasmid

#133506

Purpose

Expression of eGFP tagged PCNA to monitor DNA replication

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0478

Plasmid

#133498

Purpose

Expression of sfGFP targeted to the nucleus with two NLS signals

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(tdh1)-NLSSV40-sfGFP-NLSSV40-natMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0782

Plasmid

#133514

Purpose

Expression of sfGFP tagged LifeAct probe to monitor actin cytoskeleton

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX

Expression

Yeast

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pH3FS-PYK1

Plasmid

#85781

Purpose

N-ICE plasmid pH3FS-PYK1

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pK3F

Plasmid

#85770

Purpose

N-ICE plasmid pK3F

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWS5397-CRISPRai-NatR

Plasmid

#182836

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - NatR marker

Depositor

Rodrigo Ledesma-Amaro

Publication

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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