We narrowed to 12,482 results for: BASE

1. pENTR2B-dCas9-KRAB

   Plasmid

   #115547

   Purpose

   Entry cassette for dCas9-KRAB

   Depositor

   Rene Maehr

   Article

   Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

   Insert

   dCas9-KRAB
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. tCRMod-GGSGGS7

   Plasmid

   #111761

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS7-mRuby2 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-TRP1-PGAL1-3HA

   Plasmid

   #41609

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, TRP1 reverse orientation, and yeast ADH1 te…

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. tCRMod-GGSGGS5

   Plasmid

   #111760

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS5-mRuby2 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p1.2-Neo

   Plasmid

   #162739

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CHO Elongation factor 1A

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. ClhN-p1k-Vrg4-V5-APEX2-URA

   Plasmid

   #207071

   Purpose

   Expressing Vrg4-V5-APEX2. Marker for late Golgi/early endosomes.

   Depositor

   Zhiping Xie

   Article

   Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

   Insert

   Vrg4
   

   Use

   Tags

   V5-APEX2

   Expression

   Yeast

   Mutation

   Promoter

   pVRG4

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pgRNA-backbone

   Plasmid

   #172517

   Purpose

   Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

   Depositor

   Leonid Kruglyak

   Article

   Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

   Insert

   gRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMC-XI2

   Plasmid

   #176156

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p1.2-Hygro

   Plasmid

   #162741

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CHO Elongation factor 1A

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUS_B10

    Plasmid

    #31027

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAtU6-sgRNA

    Plasmid

    #119775

    Purpose

    Expression of a target sgRNA in potato

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    AtU6-SmR-sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-lynD3cpV

    Plasmid

    #37472

    Depositor

    Amy Palmer, Roger Tsien

    Article

    Palmer et al Chem Biol. 2006 May;13(5):521-30.

    Insert

    D3cpv second generation cameleon (calcium sensor)
    

    Use

    Tags

    the myristoylation and palmitoyla- tion sequence …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pUC-DEST-R1R2

    Plasmid

    #141056

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. At1R-P2A-mCherry

    Plasmid

    #112934

    Purpose

    Plasmid co-producing the AT1R receptor and a mCherry

    Depositor

    Dorus Gadella

    Article

    Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

    Insert

    AT1R
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-TK-Firefly-cjBCCIP

    Plasmid

    #122278

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Inserts

    Firefly luciferase
    cjBCCIP 3'UTR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC2xTC1-alphaTubulin

    Plasmid

    #36326

    Depositor

    Amy Palmer

    Article

    Van Engelenburg et al Chembiochem. 2010 Mar 1;11(4):489-93.

    Insert

    alpha tubulin 1b with tetracysteine peptides for ReAsH labeling (TUBA1B Synthetic, Human)
    

    Use

    Tags

    2xTC1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-cMSPdH45

    Plasmid

    #199767

    Purpose

    Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH45
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCO604

    Plasmid

    #139737

    Purpose

    individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnH3H

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    H3H
    

    Use

    Luciferase; Moclo level 0 component

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. VV246: CoChR(C108S)-GCaMP6f-ER in fck

    Plasmid

    #58521

    Purpose

    Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    CoChR(C108S)-GCaMP6f-ER
    

    Use

    Lentiviral

    Tags

    GCaMP6f

    Expression

    Mammalian

    Mutation

    changed Cysteine 108 to Serine

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBI

    Plasmid

    #65795

    Purpose

    Vector for iCre mRNA synthesis

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al Transgenic Res. 2013 Aug;22(4):873-5. do

    Insert

    iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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