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pENTR2b-mScarlet

Plasmid

#207961

Purpose

Gateway entry vector with the mScarlet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mScarlet

Use

Gateway entry vector

Promoter

None

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLeAPS-GFP-blast

Plasmid

#182230

Purpose

Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

GFP-P2A-Blasticidin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCK083 desmaMCS:minpromEGFP,exorh:mCherry

Plasmid

#200016

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherry

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCK011 p5E exorh

Plasmid

#195949

Purpose

Gateway p5E vector

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh regulatory elements

Use

Other

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-sfGFP 150TAG

Plasmid

#85483

Purpose

Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

superfolder GFP 150TAG

Expression

Bacterial

Mutation

150TAG (amino acid 150 mutated to stop codon)

Promoter

Arabinose

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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ND4.2-Right DdCBE-G1397-C- T1413I-GFP

Plasmid

#179686

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR

Expression

Mammalian

Mutation

T1413I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMito-MtrxRFP2

Plasmid

#172324

Purpose

fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

Depositor

Huiwang Ai

Article

Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

Insert

mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker

Tags

His-tag and Mitochondria localization sequence

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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prDNA spacer-LacOx120-20xMS2-Hyg

Plasmid

#210220

Purpose

Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

120xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FAS-GCaMP6f-WPRE

Plasmid

#216277

Purpose

AAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

GCaMP6f

Use

AAV and Cre/Lox

Tags

6XHIS and Xpress

Promoter

human synapsin 1 (hSyn)

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3

Plasmid

#90087

Purpose

Expresses wild type caspase 3 in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

Wild type

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCB59 pDEST crybb1:mKate2

Plasmid

#195986

Purpose

Transgenesis backbone

Depositor

Markus Affolter, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTriEx4-Rac1-2G

Plasmid

#66110

Purpose

Second generation Rac1 FRET biosensor

Depositor

Olivier Pertz

Article

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sp-HPV-TM(ICAM)

Plasmid

#196510

Purpose

Express HPV antigen epitope with ICAM transmembrane domain

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

a HPV epitope with CD8a stalk linker and ICAM TM domain

Use

Lentiviral

Tags

3xflag tag

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ166

Plasmid

#109328

Purpose

Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

Depositor

Yiping Qi

Article

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Insert

zCas9

Use

CRISPR; Gateway compatible zcas9 entry clone

Tags

3x FLAG, NLS and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR223-BALF4

Plasmid

#195835

Purpose

Gateway Entry Clone of Epstein-Barr virus BALF4

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BALF4 (BALF4 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDule-tfmF A65V S158A

Plasmid

#85484

Purpose

Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A

Tags

None

Expression

Bacterial

Mutation

Y32V F108W Q109M I159M with 65Val and 158Ala

Promoter

lpp (constitutive)

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

Plasmid

#114595

Purpose

Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCK004 ubi:mCerulean_SV40 (#302)

Plasmid

#195978

Purpose

Expression construct

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

ubi:mCerulean

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_HUWE1_isoform1

Plasmid

#190464

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221-NR1H2

Plasmid

#79513

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

LXR BETA (NR1H2 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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