We narrowed to 11,308 results for: ENA

Showing: 3501 - 3520 of 11308 results

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Lenti-sgKmt2d-sg3

Plasmid

#192167

Purpose

Lenti-sgKmt2d-sg3

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

Lentiviral

Mutation

NA

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G20-R20

Plasmid

#101822

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP1102

Plasmid

#119246

Purpose

knockdown phzA1 in P. aeruginosa

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA phzA1 (P. aeruginosa)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1103

Plasmid

#119247

Purpose

knockdown phzM in P. aeruginosa

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA phzM (P. aeruginosa)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1055

Plasmid

#119242

Purpose

construction intermediate

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Available Since

Dec. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-118

Plasmid

#90491

Purpose

Expresses pcDNA3 - CMVmin - 5X UAS - pFR Luciferase

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

pFR Luciferase Fluc

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1

Plasmid

#98150

Purpose

Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1

Tags

His6-TEV

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSilencer2.1-U6-NL1F35

Plasmid

#190830

Purpose

For mammalian expression of NL1F35, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

NL1F35 (A Dual-Specificity aptamer targeting OGT and GFP)

Use

Affinity Reagent/ Antibody and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

Plasmid

#170183

Purpose

Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

Depositor

Hunter Fraser

Article

Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

Insert

sgBFP

Expression

Mammalian

Promoter

hU6

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEY79

Plasmid

#191074

Purpose

flp-12 fluorescent neural reporter driving nuclear T-Sapphire expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-12 (flp-12 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY62

Plasmid

#191020

Purpose

acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-5 (acr-5 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBEAST-J23101-CocE

Plasmid

#114600

Purpose

"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

CocE

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

J23101

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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CAV1-mEGFP/FRT/TO

Plasmid

#27762

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1 (CAV1 Canis lupus familiaris)

Tags

monomeric EGFP

Expression

Mammalian

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only

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E0030 (YFP)_CD

Plasmid

#66031

Purpose

MoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Fluorescent reporter - YFP

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCS5

Plasmid

#55750

Purpose

Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Expression

Bacterial

Mutation

Residues 59-264 of KanR

Promoter

T7-lac

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf137

Plasmid

#12825

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf137

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125227

Purpose

Expresses SPARK DRD1 transmembrane component in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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Empty Anchor Control

Plasmid

#127665

Purpose

Encodes for puromycin resistance expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

Puro

Expression

Mammalian

Promoter

EF1a

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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p-mCherry2-sgC9cen

Plasmid

#198326

Purpose

Expresses a guide RNA against a repetitive locus found in the pericentromere of human chromosome 9, with mCherry2 reporter

Depositor

Sarah McClelland

Article

Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

Insert

Chr9-CEN guide RNA

Expression

Mammalian

Promoter

hU6

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_A1810

Plasmid

#91040

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

ZmUbi

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only