We narrowed to 5,103 results for: mcs

1. JDW 743 (pDONR221-EYFP-hKRAS4B-WT)

   Plasmid

   #156402

   Purpose

   Gateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codon

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human wild type KRAS4B-WT (KRAS Human)
   

   Use

   Gateway cloning

   Tags

   EYFP

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hDlx-Flex-dTomato-Fishell_7

   Plasmid

   #83894

   Purpose

   Cre recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

   Insert

   tdTomato
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   hDlx

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT151 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF10 KRAB-T2A-mCherry-BSD-WPRE

   Plasmid

   #188757

   Purpose

   Lentiviral backbone containing ZNF10 KRAB as a fusion with rTetR(SE-G72P)-3XFLAG

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   ZNF10 KRAB (ZNF10 Human)
   

   Use

   Lentiviral

   Tags

   rTetR(SE-G72P)-3xFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL1-mKalama1

   Plasmid

   #102639

   Purpose

   In vitro expression of mKalama1 from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   mKalama1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. t1-405

   Plasmid

   #166127

   Purpose

   For expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

   Depositor

   Vesa Hytönen

   Article

   Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

   Insert

   Tln1Head (1-405) (TLN1 Human)
   

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   residues 1-405 only

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMV9 lenti pEF-rTetR(SE-G72P)-3XFLAG-NOTC2(Notch)-T2A-mCherry-BSD-WPRE

   Plasmid

   #188755

   Purpose

   Lentiviral backbone containing NOTC2 Notch domain as a fusion with rTetR(SE-G72P)-3XFLAG

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   NOTC2 Notch (NOTCH2 Human)
   

   Use

   Lentiviral

   Tags

   rTetR(SE-G72P)-3xFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-puro/Flag PD-L1 4NQ

   Plasmid

   #121456

   Purpose

   expression of human PD-L1 mutant in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)

   Plasmid

   #156411

   Purpose

   pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human wild type KRAS4A-WT (KRAS Human)
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJL1-CyPet

   Plasmid

   #102636

   Purpose

   In vitro expression of CyPet from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   CyPet
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIG-836_NY-ESO-1_TCR_FAS/OX40

    Plasmid

    #207499

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/OX40, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. JDW 763 (pCS2-EYFP-hsKRAS4B-WT)

    Plasmid

    #156408

    Purpose

    pCS2 CMV expression vector driving EYFP fused human KRAS4B

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4B-WT (KRAS Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIG-833_NY-ESO-1_TCR_FAS/CD28

    Plasmid

    #207496

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/CD28, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE-RPS14(WT)-Myc

    Plasmid

    #122235

    Purpose

    expresses RPS14 protein with a Myc tag in mammalian cells (puro resistance)

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RPS14 (RPS14 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    vector's LTR

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-puro/Flag PD-L1 N200Q/N219Q

    Plasmid

    #121454

    Purpose

    expression of human PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N200Q/N219Q

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-puro/Flag PD-L1 N35Q

    Plasmid

    #121449

    Purpose

    expression of human PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N35Q

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)

    Plasmid

    #156406

    Purpose

    Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12V (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    mScarlet-I

    Mutation

    mutant KRAS4A-G12V

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

    Plasmid

    #156405

    Purpose

    Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4A-WT (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    mScarlet-I

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. t206-405

    Plasmid

    #166132

    Purpose

    For expression of human talin-1 head fragment (residues 206-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

    Depositor

    Vesa Hytönen

    Article

    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

    Insert

    T1head1-F2F3(206-405) (TLN1 Human)
    

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    residues 206-405 only

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMCB320 sgCdkn1a.1

    Plasmid

    #227945

    Purpose

    sgRNA targeting Cdkn1a, mCherry, puromycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

    Depositor

    Laura Attardi

    Article

    Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

    Insert

    Cdkn1a.1 (Cdkn1a Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMCB320 sgCdkn1a.2

    Plasmid

    #227946

    Purpose

    sgRNA targeting Cdkn1a, mCherry, puromycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

    Depositor

    Laura Attardi

    Article

    Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

    Insert

    Cdkn1a.2 (Cdkn1a Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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