We narrowed to 30,748 results for: LIS

1. pmCherry-U6-GTPBP2

   Plasmid

   #140586

   Purpose

   Expresses a gRNA and mCherry in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   gRNA GTPBP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHBDuet259

   Plasmid

   #121911

   Purpose

   NpuDnaBΔ283ΔC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   NpuDnaBΔ283ΔC39 intein
   

   Use

   Tags

   His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 283-residue endonuclease domain

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHBBAD113

   Plasmid

   #121912

   Purpose

   Charge-Introduced NpuDnaBC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   CI-NpuDnaBC39 intein
   

   Use

   Tags

   GB1-His6

   Expression

   Bacterial

   Mutation

   S110K, I111K

   Promoter

   Para

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHBBAD168

   Plasmid

   #121916

   Purpose

   Oth-NpuDnaBC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   Oth-NpuDnaBC39 intein
   

   Use

   Tags

   GB1-His6

   Expression

   Bacterial

   Mutation

   I111K, E116K

   Promoter

   Para

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBHDuet50K

   Plasmid

   #118399

   Purpose

   analysis of cis-splicing by mini inteins

   Depositor

   Hideo Iwai

   Article

   Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

   Insert

   MvuTFIIB mini intein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   incorporation of oligonucleotide during cloning

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-FAB(PX-LD)

   Plasmid

   #214422

   Purpose

   Detection of peroxisome-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   PMP34-FKBP-V5-RspAN_IRES_CFAST-20nm-Halo-6xHp NC (SLC25A17 Human)
   

   Use

   Lentiviral

   Tags

   CFAST-Halo and FKBP-V5-RspAN

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

   Plasmid

   #216250

   Purpose

   HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   HDR template for human SEC61B (SEC61B Human, Synthetic, HDR template)
   

   Use

   CRISPR; Hdr template

   Tags

   BSD.P2A.miniIAA7.3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMal_Abl-PRM 3R

   Plasmid

   #112086

   Purpose

   Bacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   Abl-PRM-3R (ABL1 Human)
   

   Use

   Tags

   His-6 and MBP

   Expression

   Bacterial

   Mutation

   construct contains only the PRM motif of Abl1

   Promoter

   Lac

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1-shCELF4-4405

   Plasmid

   #115466

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   shCELF4-4405 (CELF4 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGIPz-PD-L1 WT

    Plasmid

    #121486

    Purpose

    2nd generation lentiviral vector for over expressing human PD-L1 WT with PD-L1 shRNA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMAL-Abl-PRM 4R

    Plasmid

    #112087

    Purpose

    Bacterial expression plasmid containing His and MBP tags for 4 PRM motif repeats of Human Abl.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    Abl-PRM-4R (ABL1 Human)
    

    Use

    Tags

    His-6 and MBP

    Expression

    Bacterial

    Mutation

    construct contains only the PRM motif of Abl1

    Promoter

    Lac

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMal-Abl-PRM 5R

    Plasmid

    #112088

    Purpose

    Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    PRM-5R (ABL1 Human)
    

    Use

    Tags

    His-6 and MBP

    Expression

    Bacterial

    Mutation

    construct contains only the PRM motif of Abl1

    Promoter

    Lac

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD

    Plasmid

    #216248

    Purpose

    HDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSD

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    HDR template for human RANGAP1 (RANGAP1 Human)
    

    Use

    CRISPR and TALEN; Hdr template

    Tags

    miniIAA7.3xFlag.P2A.BSD

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1-shCELF4-4404

    Plasmid

    #115465

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shCELF4-4404 (CELF4 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX317-MBNL1

    Plasmid

    #115443

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    MBNL1 (MBNL1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216251

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgSEC61B.N1 (SEC61B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLX317-CELF4

    Plasmid

    #115445

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    CELF4 (CELF4 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-PGK-EGFP-ALDH1A2-Neo

    Plasmid

    #189743

    Purpose

    The construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A2 (ALDH1A2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. dCas9-NUP98a_IDR-EGFP

    Plasmid

    #232766

    Purpose

    Expresses dCas9-NUP98a_IDR-EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    dCas9-NUP98a_IDR-EGFP (NUP98 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBabe-Flag-SKP1-T131A

    Plasmid

    #213734

    Purpose

    Expresses human SKP1 T131A mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

    Insert

    human Flag tagged SKP1-T131A (SKP1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge