We narrowed to 3,867 results for: mak

1. NLS-R-NmeCas9 (D16A)-HA-NLS_sgRNA EZH guide

   Plasmid

   #246438

   Purpose

   All-in-one plasmid. Expresses R-NmeCas9 in mammalian cells for RNA knockdown. Spacer targeting EZH2 gene.

   Depositor

   Ailong Ke

   Article

   Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

   Inserts

   NmeCas9
   Nme-sgRNA
   

   Tags

   HA tag, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   changed Aspartic acid 16 to Alanine, deleted amin…

   Promoter

   EF-1 alpha and U6

   Available Since

   Oct. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-dual-CRISPR-U6-H1(pBP43)

   Plasmid

   #218930

   Purpose

   The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   human U6 and H1

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBMN-YFP-Parkin

   Plasmid

   #59416

   Purpose

   to make YFP-Parkin stable cell line with retrovirus

   Depositor

   Richard Youle

   Article

   Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

   Insert

   YFP-Parkin (PRKN Human)
   

   Use

   Retroviral

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-DsRed_IRES_EGFP

   Plasmid

   #92194

   Purpose

   Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   DsRed-IRES-EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM1-FLAG-metap2

   Plasmid

   #100510

   Purpose

   Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   METAP2 (METAP2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLC242-GFP-metap2

   Plasmid

   #100518

   Purpose

   Expresses GFP tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   Metap2 (METAP2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTagBFP-C1-FKBP-PIP5K1C-HD-EE

   Plasmid

   #220090

   Purpose

   Recruitable PIP5K to make PIP2

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PIP5K1C (PIP5K1C Human)
   

   Tags

   TagBFP-FKBP1A(3-108)

   Expression

   Mammalian

   Mutation

   amino acids 1-635; D101R, R304D, R445E, K446E

   Promoter

   CMV

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_OHu19986C_myc tag

   Plasmid

   #183174

   Purpose

   used to make AAV vectors for hsTRPA1 tagged with myc

   Depositor

   Sreekanth Chalasani

   Article

   Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

   Insert

   hsTRPA1 (TRPA1 Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTW040

   Plasmid

   #64769

   Purpose

   For integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminator

   Depositor

   Fred van Leeuwen

   Article

   Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

   Insert

   Cre-EBD78
   

   Tags

   fused to the human Estrogen Binding Domain (EBD),…

   Expression

   Yeast

   Promoter

   TDH3

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR_L1-YFP-Turbo-L2

    Plasmid

    #127350

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. modRNAc0-ABE8e-P2A-GFP

    Plasmid

    #178177

    Purpose

    IVT template plasmid for making ABE8e-P2A-GFP modRNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    ABE8e
    

    Use

    Ivt template plasmid

    Tags

    P2A-GFP

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBMN-mCherry-Parkin

    Plasmid

    #59419

    Purpose

    to make mCherry-Parkin stable cell line with retrovirus

    Depositor

    Richard Youle

    Article

    Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

    Insert

    mCherry-Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. cASPG1-mRFP

    Plasmid

    #181962

    Purpose

    ASPG1 protein tagged with mRFP, under control of TBA2 promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    ASPG1 (ASPG1 Mustard Weed)
    

    Tags

    mRFP

    Expression

    Plant

    Promoter

    TBA2 promoter

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CROPseq-Guide-BSD

    Plasmid

    #216123

    Purpose

    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR_L1-YFP-miniTurbo-L2

    Plasmid

    #127352

    Purpose

    gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. neurod1:cre; cmlc2:EGFP

    Plasmid

    #173480

    Purpose

    cre is driven by -5kb neurod1 promoter. The vector contain a cmlc2:EGFP maker

    Depositor

    John Rawls

    Article

    Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

    Insert

    Cre
    

    Use

    Zebrafish expression

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSS146

    Plasmid

    #64770

    Purpose

    For integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminator

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    Cre-EBD78
    

    Tags

    fused to the human Estrogen Binding Domain (EBD),…

    Expression

    Yeast

    Promoter

    TDH4

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR_L1-YFP-miniTurbo-NES-L2

    Plasmid

    #127351

    Purpose

    gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR_L1-YFP-Turbo-NES-L2

    Plasmid

    #127349

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB-CAG-FLEX-F14F15-BFP-(mCherry)'

    Plasmid

    #201831

    Purpose

    piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Inserts

    CAG
    BFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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