We narrowed to 5,110 results for: mcs

1. pCDH-puro/Flag PD-L1 N35Q

   Plasmid

   #121449

   Purpose

   expression of human PD-L1 mutant in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N35Q

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)

   Plasmid

   #156406

   Purpose

   Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codon

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12V (KRAS Human)
   

   Use

   Gateway cloning

   Tags

   mScarlet-I

   Mutation

   mutant KRAS4A-G12V

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

   Plasmid

   #156405

   Purpose

   Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human wild type KRAS4A-WT (KRAS Human)
   

   Use

   Gateway cloning

   Tags

   mScarlet-I

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. t206-405

   Plasmid

   #166132

   Purpose

   For expression of human talin-1 head fragment (residues 206-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

   Depositor

   Vesa Hytönen

   Article

   Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

   Insert

   T1head1-F2F3(206-405) (TLN1 Human)
   

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   residues 206-405 only

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCB320 sgCdkn1a.1

   Plasmid

   #227945

   Purpose

   sgRNA targeting Cdkn1a, mCherry, puromycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

   Depositor

   Laura Attardi

   Article

   Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

   Insert

   Cdkn1a.1 (Cdkn1a Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMCB320 sgCdkn1a.2

   Plasmid

   #227946

   Purpose

   sgRNA targeting Cdkn1a, mCherry, puromycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

   Depositor

   Laura Attardi

   Article

   Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

   Insert

   Cdkn1a.2 (Cdkn1a Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX304-KDM6A-M1190T/E1335D

   Plasmid

   #203581

   Purpose

   Expresses mutant version of KDM6A with increased oxygen affinity in mammalian cells.

   Depositor

   Abhishek Chakraborty, William Kaelin

   Article

   Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

   Insert

   KDM6A (KDM6A Human)
   

   Use

   Lentiviral

   Tags

   HA-tagged

   Expression

   Mammalian

   Mutation

   Changed Methionine 1190 Threonine and Glutamic Ac…

   Promoter

   CMV

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgMST1/2-2

   Plasmid

   #229430

   Purpose

   knockout of MST1 and MST2

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgMST1/2-2 (MST1, STK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6;bU6;EFS

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgMST1/2-1

   Plasmid

   #229429

   Purpose

   knockout of MST1 and MST2

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgMST1/2-1 (MST1, STK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6;bU6;EFS

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v5 (B.1.617.3)

    Plasmid

    #212540

    Purpose

    Encodes SARS-CoV-2 spike T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D614G,P681R,D950N for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D614G,P681R,D950N (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTwist-SARS-CoV-2 Spike-deltaC18 BA.4

    Plasmid

    #212519

    Purpose

    Encodes SARS-CoV-2 variant BA.4 Spike for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike BA.4 (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    T19I ,deltaL24P25P26 ,A27S ,delH69-V70 ,G142D ,V2…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA) G496S (TCA) Q498R (CGA) N501Y (TAT) Y505H (CAT)

    Plasmid

    #212477

    Purpose

    Encodes SARS-CoV-2 spike Q493R, G496S, Q498R, N501Y, Y505H for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike Q493R, G496S, Q498R, N501Y, Y505H (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    Q493R, G496S, Q498R, N501Y, Y505H

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTwist-SARS-CoV-2 Spike-deltaC18 AY.1

    Plasmid

    #212433

    Purpose

    Encodes SARS-CoV-2 spike T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTwist-SARS-CoV-2 Spike-deltaC18 AY.2

    Plasmid

    #212434

    Purpose

    Encodes SARS-CoV-2 spike T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v2 (B.1.617.1 v2)

    Plasmid

    #212429

    Purpose

    Encodes SARS-CoV-2 spike T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E484Q,D614G,P681R,Q1071H for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E484Q,D614G,P681R,Q1071H (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTwist-SARS-CoV-2 Spike-deltaC18 SAK006879 VOC-501Y.V2 (B.1.351v5)

    Plasmid

    #212391

    Purpose

    Encodes SARS-CoV-2 spike D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTwist-SARS-CoV-2 Spike-deltaC18 SAK007134 VOC-501Y.V2 (B.1.351v6)

    Plasmid

    #212393

    Purpose

    Encodes SARS-CoV-2 spike A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N5…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTwist-SARS-CoV-2 Spike-deltaC18 (P.1) - No RBD Mutations

    Plasmid

    #212384

    Purpose

    Encodes SARS-CoV-2 spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V11…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTwist-SARS-CoV-2 Spike-deltaC18 SA190 VOC-501Y.V2 (B.1.351v4)

    Plasmid

    #212389

    Purpose

    Encodes SARS-CoV-2 spike D80A,D215G,L242-L244 deletion,E484K,N501Y,D614G,A701V for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike D80A,D215G,L242-L244 deletion,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    D80A,D215G,L242-L244 deletion,E484K,N501Y,D614G,A…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTwist-SARS-CoV-2 Spike-deltaC18 VOC-501Y.V2 (B.1.351) - All Mutations

    Plasmid

    #212381

    Purpose

    Encodes SARS-CoV-2 spike L18F,D80A,D215G,L242H,A243I,Q414N,E484K,N501Y,D614G,A701V for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike L18F,D80A,D215G,L242H,A243I,Q414N,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    L18F,D80A,D215G,L242H,A243I,Q414N,E484K,N501Y,D61…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge