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We narrowed to 15,796 results for: eng

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Showing: 3541 - 3560 of 15796 results

  1. pRVL-3

    Plasmid
    #104581
    Purpose
    Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Publication
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and E318A/K320A/K322A
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    104581-plasmid-map-sequence-id-201392

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  5. MCP-MSN

    Plasmid
    #210704
    Purpose
    This plasmid express EF1a promoter driven MCP-MSN transactivation module
    Depositor
    Isaac Hilton
    Publication
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2 (STAT1, MRTFA Human, bacteriophage MS2, Synthetic)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    210704-plasmid-map-sequence-id-416247

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  10. pKAR2-Br512g3.2

    Plasmid
    #171201
    Purpose
    Expresses Br512g3.2 polymerase in E.coli
    Depositor
    Andrew Ellington
    Publication
    Bhadra et al medRxiv 2022.08.09.22278605
    Insert
    Engineered Bst DNA Polymerase I Large Fragment
    Tags
    8x His tag; Villin Headpiece 47 amino acid (vHP47…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171201-plasmid-map-sequence-id-339558

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  11. pET30a-His6-ABP-LOXCAT

    Plasmid
    #139971
    Purpose
    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).
    Depositor
    Vamsi Mootha
    Publication
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    139971-plasmid-map-sequence-id-311161

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  13. GLI2 his

    Plasmid
    #87097
    Purpose
    expresses GLI2 in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Insert
    GLI2 (GLI2 Human)
    Tags
    6x His, T7 tag, and Xpress tag
    Expression
    Mammalian
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    87097-plasmid-map-sequence-id-201477

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  18. gGBEv6.3-SpG

    Plasmid
    #202630
    Purpose
    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.
    Insert
    nSpG(D10A)-MPGv6.3
    Expression
    Mammalian
    Mutation
    SpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…
    Promoter
    hU6, EF1a, CBh
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202630-plasmid-map-sequence-id-398429

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Showing: 3541 - 3560 of 15796 results