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783-Rx-mU6: RfxCas13d gRNA and array cloning backbone

Plasmid

#228361

Purpose

mU6-driven expression of RfxCas13d gRNAs and arrays. Contains AarI sites for guide cloning.

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Rfxcas13d grna expression …

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

Plasmid

#60229

Purpose

Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Cre recombinase

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA

Expression

Mammalian

Promoter

CBh and U6

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pNP2314 [Cloning backbone, Gluc/Cluc Inversion Activity Reporter]

Plasmid

#247248

Purpose

Cloning backbone for measuring bridge recombinase mediated inversion

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)

Plasmid

#238184

Purpose

Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF5 (KLF5 Human, Synthetic)

Use

Lentiviral

Tags

mTagBFP2

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMB1: All in one SpCas9 expression and sgRNA cloning backbone

Plasmid

#228363

Purpose

mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone

Plasmid

#228362

Purpose

mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Rfxcas13d grna expression …

Expression

Mammalian

Promoter

mU6 and hU6

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)

Plasmid

#238181

Purpose

Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF5 (KLF5 Human, Synthetic)

Use

Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR

Plasmid

#68347

Purpose

AAV plasmid expressing the FlpO recombinase. with empty gRNA

Depositor

Jannik Elverløv-Jakobsen

Insert

FlPO

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn syph-GFP-myc-BoNT/B(1-146)-iLID

Plasmid

#122981

Purpose

AAV plasmid with human synapsin promoter driving synaptophysin fused to GFP, iLID(V416I) and BoNT/B amino acids 1-146. Co-express with SSPB-BoNT(147-441, Y365A) for vPA-BoNT

Depositor

Matthew Kennedy

Article

Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

Insert

Syph-GFP-myc-BoNT/B(1-146)-iLID (Syp Rat, Synthetic)

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pXTK-BB7 [Backbone_repABC-pMlb-KanR-TetR-PaqCI-Compatible_PartTypeBB]

Plasmid

#229393

Purpose

XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_repABC-pMlb-KanR-TetR-PaqCI-Compatible_PartTypeBB and an sfGFP dropout

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Type

Empty backbone

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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