We narrowed to 1,804 results for: FAST

1. pTS2385-Tier1-PhCMV-fastFT

   Plasmid

   #169518

   Purpose

   Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT (PhCMV-fastFT-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven fluorescent timer, fast maturation time
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFASTbac-HisGST-PTPIP51(36-470)

   Plasmid

   #170531

   Purpose

   Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 )
   

   Tags

   GST tag and His tag

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac-LacI-LAP-HEC1

   Plasmid

   #114033

   Purpose

   Production of baculovirus with LacI-LAP-HEC1

   Depositor

   Geert Kops

   Article

   Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

   Insert

   LacI-LAP-HEC1 (NDC80 Human)
   

   Expression

   Insect

   Mutation

   none

   Promoter

   CMV

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac(His10-Lckg2a,Y394F)

   Plasmid

   #177893

   Purpose

   Baculo expression of His10 tag fused to human LckG2A

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   Lck (LCK Human)
   

   Tags

   10xHis-TEV

   Expression

   Insect

   Mutation

   G2A,Y394F

   Promoter

   Polyhedrin

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac-M13-6His-KIF1C-GFP

   Plasmid

   #130975

   Purpose

   Expression of 6His-KIF1C-GFP in insect cells for protein purification.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

   Insert

   KIF1C-GFP (KIF1C Human)
   

   Tags

   6His and GFP

   Expression

   Insect

   Mutation

   5 silent mutations that make this construct RNAi …

   Promoter

   Polyhedrin

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac-GFP-dynein-2(D1091–Q4307)

   Plasmid

   #64064

   Purpose

   Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

   Depositor

   Andrew Carter

   Article

   Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

   Insert

   Cytoplasmic dynein-2 (DYNC2H1 Human)
   

   Tags

   GFP, His tag, and ZZ tag

   Expression

   Insect

   Mutation

   Deleted amino acids 1-1090, extra C-terminal vali…

   Promoter

   Polyhedrin

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-His-MBP-SCov2-nsp12

   Plasmid

   #154759

   Purpose

   Expression of SARS-CoV-2 nsp12

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Insect

   Promoter

   Polyhedron

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac Dual α1B/β2B Tubulin

   Plasmid

   #170553

   Purpose

   Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

   Depositor

   Tarun Kapoor

   Article

   Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

   Inserts

   TUBA1B (TUBA1B Human)
   TUBB2B (TUBB2B Human)
   

   Tags

   AP linker, GS linker (GGSGG), His10 tag, L21 enha…

   Expression

   Insect

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. ER-mit RspA-splitFAST (lentiviral plasmid)

   Plasmid

   #233604

   Purpose

   Expression of ER-mit RspA-splitFAST by a single lentiviral plasmid encoding the two fragments

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   RspA-splitFAST
   

   Use

   Lentiviral

   Promoter

   CMV/SV40

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET51b-His-TEV-SNAP-tag_fast

    Plasmid

    #167271

    Purpose

    Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac Dual LIC cloning vector (5A)

    Plasmid

    #30121

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFAST-BAC BAF155/SMARCC1-His

    Plasmid

    #177863

    Purpose

    Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

    Depositor

    Yoshihiro Izumiya

    Article

    Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

    Insert

    SMARCC1 (SMARCC1 Human)
    

    Tags

    His and His tag at C-terminus with GGGGS linker

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

    Plasmid

    #130976

    Purpose

    Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    Hook3-SNAPf (HOOK3 Human)
    

    Tags

    8His-ZZ-LTLT and SNAPf

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET51b-His-TEV-CLIP-tag_fast

    Plasmid

    #167275

    Purpose

    Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    CLIPf-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFAST-BAC BAF57/SMARCE1-His

    Plasmid

    #177861

    Purpose

    Transfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His protein

    Depositor

    Yoshihiro Izumiya

    Article

    Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

    Insert

    SMARCE1 (SMARCE1 Human)
    

    Tags

    His tag at C-terminus with GGGGS linker

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid

    #216377

    Purpose

    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)

    Depositor

    William Weis

    Insert

    LDL receptor related protein 6 (LRP6 Human)
    

    Use

    Baculovirus

    Tags

    9xHis and HA signal sequence-FLAG-3C cleavage sit…

    Expression

    Insect

    Promoter

    Polyhedrin (LRP6)/p10 (MESD)

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac-Dual- GST-TEV-TBK1

    Plasmid

    #208875

    Purpose

    For expression in insect cells of TBK1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    TBK1 (TBK1 Human)
    

    Tags

    GST-TEV

    Expression

    Insect

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac (GST-preSci site-CD45)

    Plasmid

    #177889

    Purpose

    Baculo expression of GST tag fused to human CD45 ICD

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    CD45 (aa 598-1304 only) (PTPRC Human)
    

    Tags

    TEV-GST-PreScission cut site

    Expression

    Insect

    Mutation

    L652P

    Promoter

    Polyhedrin

    Available Since

    Aug. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWZL Neo Myr Flag FASTK

    Plasmid

    #20485

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    FASTK (FASTK Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pFastBac1-OSF-TRIM5alpha(human)(R332P)

    Plasmid

    #79037

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(human R332P) (TRIM5 Human)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Changed Arg 332 to Pro

    Promoter

    polyhedrin

    Available Since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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