We narrowed to 1,917 results for: FRT

1. pcDNA5-FRT-TO-Nsp14-HF

   Plasmid

   #157719

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp14-HF (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pcDNA5FRT/TO-Cezanne-Strep-HA

   Plasmid

   #113492

   Purpose

   Expression of human Cezanne with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   Cezanne (OTUD7B Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5/FRT/TO_HaloTag7_SKL

   Plasmid

   #175532

   Purpose

   Peroxysomal expression of HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag7-SKL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT/TO HSPH2

   Plasmid

   #19454

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 4 (HSPA4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutations A381T, T732A, and an insertion of "…

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCol1a1-frt (FVB)

   Plasmid

   #63575

   Purpose

   Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.

   Depositor

   Ivo Huijbers

   Article

   Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

   Insert

   Col1A-frt-hygro-pA
   

   Use

   Mouse Targeting; Frt/flpe

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   None

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-FRT-TO-eGFP-HF

   Plasmid

   #157735

   Purpose

   mammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   eGFP-HF
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5/FRT/TO V5 DNAJB8

   Plasmid

   #19531

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA-FRT-FAK10

   Plasmid

   #59119

   Purpose

   FAK fret sensor with 10 nm helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Use

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5-FRT-TO-Nsp12

   Plasmid

   #157709

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp12 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pcDNA5-FRT-TO-FH-Nsp12

    Plasmid

    #157710

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp12 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pcDNA5-FRT/TO-V5-SHLD3

    Plasmid

    #114124

    Purpose

    Expresses N-terminally tagged V5-SHLD3 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD3 (SHLD3 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available Since

    Aug. 23, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pcDNA5-FRT-TO-Nsp8

    Plasmid

    #157694

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pcDNA5/FRT/TO C1-APEX2

    Plasmid

    #129641

    Purpose

    Express ER-localized C1-APEX2 fusion in mammalian cells

    Depositor

    Nicholas Ingolia

    Article

    Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

    Insert

    C1(1-29)-APEX2-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5/FRT/TO V5 DNAJB7

    Plasmid

    #19530

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 7 (DNAJB7 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5FRT/TO-Ataxin3-Strep-HA

    Plasmid

    #113496

    Purpose

    Expression of human Ataxin-3 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    Ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5/FRT/TO DNAJB3

    Plasmid

    #19471

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB3 (Dnajb3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO DNAJB4

    Plasmid

    #19472

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB4 (DNAJB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5FRT/TO-SVIP-Strep-HA

    Plasmid

    #113491

    Purpose

    Expression of human SVIP with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    SVIP (SVIP Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5FRT/TO-RNF185-GFP

    Plasmid

    #113498

    Purpose

    Expression of human RNF185 with C-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    RNF185 (RNF185 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5/FRT-ChemoG1

    Plasmid

    #193806

    Purpose

    CMV overexpression of ChemoG1 in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG1
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    EGFP: A206K

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge