We narrowed to 1,873 results for: FRT

1. FRT-acrymCherry-FRT

   Plasmid

   #241179

   Purpose

   Middle entry vector for mini-Golden subcloning platform; acry-mCherry

   Depositor

   Junsu Kang

   Insert

   acry-mCherry
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   TCGC linker

   Promoter

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

   Plasmid

   #52889

   Purpose

   "CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

   Depositor

   Iswar Hariharan

   Article

   Bosch et al Development. 2015 Feb 1;142(3):597-606.

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

   Plasmid

   #52890

   Purpose

   "CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

   Depositor

   Iswar Hariharan

   Article

   Bosch et al Development. 2015 Feb 1;142(3):597-606.

   Inserts

   LexGAD
   Gal4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

   Plasmid

   #175544

   Purpose

   Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   IgKchL_HA-HaloTag9-myc_PDGFRtmb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

   Plasmid

   #175534

   Purpose

   Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   IgKchL_HA-HaloTag7-myc_PDGFRtmb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

   Plasmid

   #182009

   Purpose

   CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   IgKchL_HA_STf_myc_PDGFRtmb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. tubP-FRT>GAL80-6-FRT>

   Plasmid

   #63173

   Purpose

   ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "Flp-Out" GAL80
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3E-FRT-KanR-FRT pA

   Plasmid

   #82601

   Purpose

   3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   FRT-Kan-FRT
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-sfGFP-pA-FRT-Amp-FRT

   Plasmid

   #176620

   Purpose

   for generating PCR templates with sfGFP and Amp for BAC recombination

   Depositor

   Nathan Lawson

   Article

   Shih et al Development. 2021 Nov 9. pii: 273393. do

   Insert

   super folder green fluorescent protein with FRT-flanked ampicillin cassette
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   H217R - please see dep. comments

   Promoter

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1

    Plasmid

    #205152

    Purpose

    Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    FRT-GFP-FRT-uORF-dCBEevoCDA1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAST

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. UAS-FRT-GFP-FRT-u(M)Cas9

    Plasmid

    #127388

    Purpose

    Cas9 inducible by removal of a GFP flip-out cassette

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    FRT-GFP-FRT-uORF(M)Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 78H05-FRT100-STOP-FRT100-p65.AD

    Plasmid

    #232828

    Purpose

    Enhancer 78H05 driven p65.AD, gated by Sparse.FRT100

    Depositor

    Liqun Luo

    Article

    Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

    Inserts

    FRT100-STOP-FRT100
    p65.AD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMC-ME_CreER-pA-FRT-acry:venus-pA-FRT

    Plasmid

    #200543

    Purpose

    mini circle middle entry plasmid for CreER-pA-FRT-acry:venus-pA-FRT with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    CreER
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMC-ME_CreER-pA-FRT-acry:mCherry-pA-FRT

    Plasmid

    #200544

    Purpose

    mini circle middle entry plasmid for CreER-pA-FRT-acry: mCherry -pA-FRT with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    CreER, mCherry
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. VT028327-FRT10-STOP-FRT10-p65.AD

    Plasmid

    #232827

    Purpose

    Enhancer VT028327 driven p65.AD, gated by Sparse.FRT10

    Depositor

    Liqun Luo

    Article

    Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

    Inserts

    FRT10-STOP-FRT10 (stop )
    p65.AD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. VT028327-FRT10-STOP-FRT10-GAL4

    Plasmid

    #232829

    Purpose

    Enhancer VT028327 driven GAL4, gated by Sparse.FRT10

    Depositor

    Liqun Luo

    Article

    Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

    Inserts

    FRT10-STOP-FRT10
    GAL4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-p2A-CreERT2-FRT-kan-FRT

    Plasmid

    #108454

    Purpose

    This plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.

    Depositor

    Nadia Mercader Huber

    Article

    Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

    Insert

    mCherry-p2a-CreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. iF65 PB-FrtStopFrt-Zim3-dCas9-mScarlet-hygro

    Plasmid

    #204730

    Purpose

    Frt-inducible CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQUAST-frt-stop-frt-p65AD::CaM

    Plasmid

    #64716

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Inserts

    p65AD::CaM
    frt-stop-frt
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM

    Plasmid

    #71811

    Purpose

    Generating transgenic flies for FLP-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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