We narrowed to 3,266 results for: actin

1. pBactin-Nedd1-mOrange2-rCM1

   Plasmid

   #196861

   Purpose

   Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-rCM1 (Nedd1 Rat)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBactin-Nedd1-mOrange2-F75A

   Plasmid

   #196862

   Purpose

   Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-F75A (Nedd1 Rat)
   

   Expression

   Mammalian

   Mutation

   F75A in the CM1 domain

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BAF53b + GFP/p-Actin-IRES

   Plasmid

   #197297

   Purpose

   Plasmid expressing an antigen targeting BAF53b

   Depositor

   Jerry Crabtree

   Insert

   BAF53b (Actl6b Mouse)
   

   Tags

   GFP (GFP observed)

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBetaActin-KIF1aMotor-mScarlet-FKBP

   Plasmid

   #191336

   Purpose

   Expresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   KIF1aMotor-mScarlet-FKBP (Kif1a Mouse)
   

   Expression

   Mammalian

   Mutation

   motor domain aa 1-405

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBetaActin-FKBP-Halo-Dync1h1motor

   Plasmid

   #191333

   Purpose

   Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FKBP-Halo-Dync1h1motor (Dync1h1 Mouse)
   

   Expression

   Mammalian

   Mutation

   DYNC1H1 motor domain aa 1453-4644

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBetaActin-FRB-dCer3-CAAX

   Plasmid

   #191326

   Purpose

   Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FRB-dCer-CAAX
   

   Expression

   Mammalian

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBetaActin-FKBP-mScarlet-KIFC1motorN593K

   Plasmid

   #191335

   Purpose

   Expresses the fluorescently labeled minus-end directed KIFC1 motor-deficient motor domain fused to FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FKBP-mScarlet-KIFC1N593K (KIFC1 Human)
   

   Expression

   Mammalian

   Mutation

   motor domain aa 125-673; N593K

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T

   Plasmid

   #191331

   Purpose

   Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
   

   Expression

   Mammalian

   Mutation

   DYNC1H1 motor domain aa 1453-4644

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PA-mCherry-miniSOG-Alpha-Actinin-19

   Plasmid

   #57782

   Purpose

   Localization: Alpha Actinin, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Alpha-Actinin (ACTN1 Human)
   

   Tags

   PA-mCherry-miniSOG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

10. YFP NLS Beta-Actin G13R

    Plasmid

    #60615

    Purpose

    Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    G13R

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid

    #58475

    Purpose

    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. YFP NLS Beta-Actin S14C

    Plasmid

    #60614

    Purpose

    Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    S14C

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Reduced Expression GFP beta actin

    Plasmid

    #31502

    Depositor

    Rick Horwitz, Tim Mitchison

    Article

    Watanabe et al Science. 2002 Feb 8. 295(5557):1083-6.

    Insert

    beta actin (ACTB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    91-544 of enhancer region of CMV promoter deleted

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-FLAG-Tev_ACTB (beta-actin)

    Plasmid

    #188453

    Purpose

    Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    ACTB (ACTB Human)
    

    Tags

    6xHis-FLAG

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2

    Plasmid

    #191338

    Purpose

    Expresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice cultures

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    TUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)
    

    Expression

    Mammalian

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Actin Binding Domain Tension Sensor

    Plasmid

    #215368

    Purpose

    Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    Actin Binding Domain Tension Sensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Actin Binding Domain Tensionless Control

    Plasmid

    #215371

    Purpose

    Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    Actin Binding Domain Tensionless Control
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBactin-AcGFP-128-425-Akap9

    Plasmid

    #196871

    Purpose

    Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to AcGFP. Used to displace endogenous Akap9 from Golgi

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP-Akap9 (128-425) (Akap9 Rat)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDestTol2-actinb-kcna1a-IRES-EGFP

    Plasmid

    #164965

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcna1a (kcna1a Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Promoter

    actinb (actb2) promoter

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBac{3XP3::DsRed, Pactin::Ptrsps}

    Plasmid

    #86862

    Purpose

    Expresses DsRed in eyes and ubiquitous expression of P-transposase

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    pACNNTNPII-S129A
    

    Expression

    Insect

    Mutation

    S129A

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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