We narrowed to 6,202 results for: cas9 expression plasmid

1. Nme2Cas9_pMCSG7

   Plasmid

   #119925

   Purpose

   Nme2Cas9 bacterial expression plasmid

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   Tags

   NLS, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nme2Cas9_pCDest

   Plasmid

   #119923

   Purpose

   Nme2Cas9 CMV-driven mammalian expression plasmid

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   Tags

   NLS-3xHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9GG

   Plasmid

   #131009

   Purpose

   Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Inserts

   direct repeat of SpCas9
   promoter PJ23119
   mRFP expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_R0040 TetR

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. EC2_2_dCas9_VP64_sgRNA

   Plasmid

   #163707

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VP64+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9-J23109

   Plasmid

   #113150

   Purpose

   Plasmid constitutively expressing Cas9 protein

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

   Plasmid

   #139987

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS

   Plasmid

   #121821

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9 (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. IH601: pMAGIC (R4-R3) NLS-Sa Cas9-NLS

   Plasmid

   #121827

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS SaCas9 (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-Cas9 (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdCas9-omega

    Plasmid

    #114632

    Purpose

    Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

    Depositor

    Anushree Chatterjee

    Article

    Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

    Insert

    dCas9-omega
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

    Plasmid

    #71707

    Purpose

    A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

    Depositor

    Giulio Draetta

    Article

    Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

    Insert

    humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
    

    Use

    CRISPR

    Tags

    3xFLAG and hGEM(1/110)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMA-SpCas9-g3

    Plasmid

    #80786

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMA-SpCas9-g4

    Plasmid

    #80787

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMA-SpCas9-g1

    Plasmid

    #80784

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMA-SpCas9-g2

    Plasmid

    #80785

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMA-SpCas9-g5

    Plasmid

    #80788

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMA-SpCas9-g6

    Plasmid

    #80789

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMA-SpCas9-g7

    Plasmid

    #80790

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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