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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,065 results for: gibson assembly

Showing: 341 - 360 of 2065 results

  1. U6m

    Plasmid
    #121019
    Purpose
    MoClo golden gate assembly BC part for BCD20 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD20
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121019-plasmid-map-sequence-id-238082

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  2. U7m

    Plasmid
    #121020
    Purpose
    MoClo golden gate assembly BC part for BCD16 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD16
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121020-plasmid-map-sequence-id-236180

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  3. U8m

    Plasmid
    #121021
    Purpose
    MoClo golden gate assembly BC part for BCD24 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD24
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121021-plasmid-map-sequence-id-236184

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  4. C84m

    Plasmid
    #120995
    Purpose
    MoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma 28
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120995-plasmid-map-sequence-id-236105

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  5. C95m

    Plasmid
    #121001
    Purpose
    MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7 RNA polymerase (Andy please check!)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121001-plasmid-map-sequence-id-236130

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  6. C63m

    Plasmid
    #120979
    Purpose
    MoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    lacI
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120979-plasmid-map-sequence-id-236033

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  7. C3m

    Plasmid
    #120956
    Purpose
    MoClo golden gate assembly CD part for sfGFP (Superfolder green fluorescent protein from DOI: 10.1038/nbt1172; slight variant on https://www.fpbase.org/protein/superfolder-gfp/)
    Depositor
    Richard Murray
    Insert
    sfGFP
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120956-plasmid-map-sequence-id-235970

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  8. P30m

    Plasmid
    #120942
    Purpose
    MoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pL-lacO1
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120942-plasmid-map-sequence-id-238038

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  9. MZB184

    Plasmid
    #228488
    Purpose
    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228488-plasmid-map-sequence-id-458447

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  10. MZB223

    Plasmid
    #228491
    Purpose
    Empty C-terminal 8xHis tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    8xHis
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228491-plasmid-map-sequence-id-455392

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  11. MZB116

    Plasmid
    #228480
    Purpose
    "Empty" C-terminal self-cleaving CPD-tag vector for rapid cloning/DNA Assembly via SwaI restriction enzyme site (bacterial expression)
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    CPD-10xHis
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    228480-plasmid-map-sequence-id-455378

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  12. MZB133

    Plasmid
    #228484
    Purpose
    "Empty" N-terminal TEV cleavable 6x His tag vector for rapid cloning/DNA Assembly via SmaI restriction site (Bacterial Expression)
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228484-plasmid-map-sequence-id-457332

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  13. C77m

    Plasmid
    #120989
    Purpose
    MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AmpR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120989-plasmid-map-sequence-id-236056

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  14. C78m

    Plasmid
    #120990
    Purpose
    MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ChlorR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120990-plasmid-map-sequence-id-236059

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  15. p006-GFP-pBAD

    Plasmid
    #108315
    Purpose
    GFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    GFP under control of arabinose-inducible promoter pBAD
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    108315-plasmid-map-sequence-id-218821

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  18. p005-RFP-strong

    Plasmid
    #108317
    Purpose
    Synthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    RFP under controlf of strong synthetic constitutive promoter pFAB4005
    Expression
    Bacterial
    Promoter
    pFAB4005
    Available Since
    Aug. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    108317-plasmid-map-sequence-id-218823

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Showing: 341 - 360 of 2065 results