We narrowed to 2,170 results for: gibson assembly
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Plasmid#229960PurposebigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloningDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only
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psBIG1e
Plasmid#229961PurposebigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloningDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
psBIG1e
Plasmid#229961PurposebigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloningDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
psBIG1c
Plasmid#229959PurposebigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloningDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
psBIG1c
Plasmid#229959PurposebigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloningDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
C80m
Plasmid#120992PurposeMoClo golden gate assembly CD part for mfLon protease (see doi: [10.1038/nbt.3053]). Please see Supplemental Documents for annotated Genbank file.DepositorInsertmfLon protease
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C80m
Plasmid#120992PurposeMoClo golden gate assembly CD part for mfLon protease (see doi: [10.1038/nbt.3053]). Please see Supplemental Documents for annotated Genbank file.DepositorInsertmfLon protease
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C86m
Plasmid#120997PurposeMoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.DepositorInsertX174E
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C86m
Plasmid#120997PurposeMoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.DepositorInsertX174E
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C11m
Plasmid#120959PurposeMoClo golden gate assembly CD part for Citrine (modified YFP; variant of https://www.fpbase.org/protein/citrine/) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertCitrine
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C11m
Plasmid#120959PurposeMoClo golden gate assembly CD part for Citrine (modified YFP; variant of https://www.fpbase.org/protein/citrine/) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertCitrine
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C5m
Plasmid#120957PurposeMoClo golden gate assembly CD part for mRFP (monomeric red fluorescent protein; see https://doi.org/10.1073/pnas.082243699 and https://www.fpbase.org/protein/mrfp1/).DepositorInsertmRFP
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C5m
Plasmid#120957PurposeMoClo golden gate assembly CD part for mRFP (monomeric red fluorescent protein; see https://doi.org/10.1073/pnas.082243699 and https://www.fpbase.org/protein/mrfp1/).DepositorInsertmRFP
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C115m
Plasmid#121014PurposeMoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.DepositorInsertTP901 integrase
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C115m
Plasmid#121014PurposeMoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.DepositorInsertTP901 integrase
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U22m
Plasmid#121025PurposeMoClo golden gate assembly BC part (high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD6
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U22m
Plasmid#121025PurposeMoClo golden gate assembly BC part (high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD6
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C32m
Plasmid#120967PurposeMoClo golden gate assembly CD part for CinR (Quorum-sensing receptor for 3OC14). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohCD carb CinR
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C36m
Plasmid#120970PurposeMoClo golden gate assembly CD part for RhlI (quorum-sensing synthase for 3OC14). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohCD carb RhlI
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C40m
Plasmid#120973PurposeMoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertBlank (Random 50bp sequence)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only