We narrowed to 6,722 results for: guide rna expression plasmid

1. p(U6b-BsaI-gRNA)

   Plasmid

   #65956

   Purpose

   Tribolium U6b promoter with BsaI cloning site to insert guide RNA sequence

   Depositor

   Michalis Averof

   Article

   Gilles et al Development. 2015 Jul 9. pii: dev.125054

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-puro U6 sgRNA BfuAI large stuffer

   Plasmid

   #52628

   Purpose

   lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency

   Depositor

   Scot Wolfe

   Insert

   Kanamycin cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Wei lab paired-guide RNA (pgRNA) CRISPR–Cas9 library for human long non-coding RNAs (lncRNAs)

   Pooled Library

   #89640

   Purpose

   Paired sgRNA library containing 12,472 pgRNAs targeting 671 human long non-coding RNAs (lncRNAs)

   Depositor

   Wensheng Wei

   Article

   Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

   Expression

   Mammalian

   Use

   Lentiviral

   Available since

   May 17, 2017

   Availability

   Academic Institutions and Nonprofits only

4. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60226

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCJH009_proD_Cas12c-sgRNA-GFP_CAM

   Plasmid

   #183070

   Purpose

   Plasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c single guide RNA targeting GFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. gRNA-hIRF-1 #12/pSIR-hCD2

   Plasmid

   #135392

   Purpose

   Expresses a guide RNA (gRNA) to target human IRF-1 promoter

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   gRNA_hIRF1 promoter #12
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

   Plasmid

   #73796

   Purpose

   pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

   Depositor

   Ronald Davis

   Article

   Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

   Inserts

   dCas9-Mxi1
   Tet Repressor
   Structural gRNA for S pyogenes
   

   Use

   CRISPR

   Tags

   Mxi1 and NLS

   Expression

   Yeast

   Mutation

   D10A, H840A

   Promoter

   pGPM1, pRPR1(TetO), and pTef1

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SaCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122090

   Purpose

   U6 driven SaCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

   Plasmid

   #60231

   Purpose

   Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   EGFP
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA-P2A and EGFP-KASH

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hSyn

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p(U6a-BsaI-gRNA)

    Plasmid

    #65955

    Purpose

    Tribolium U6a promoter with BsaI cloning site to insert guide RNA sequence

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2015 Jul 9. pii: dev.125054

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p(U6b-BsaI-gRNA)

    Plasmid

    #65956

    Purpose

    Tribolium U6b promoter with BsaI cloning site to insert guide RNA sequence

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2015 Jul 9. pii: dev.125054

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. px552-U6-gRNA1-CMV-eGFP

    Plasmid

    #211758

    Purpose

    sgRNA1 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

    Depositor

    Glenn Yiu

    Article

    Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

    Insert

    gRNA1 targeting VEGF-A (VEGFA Mouse, M. mulatta (rhesus macaque), Human)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. px552-U6-gRNA2-CMV-eGFP

    Plasmid

    #211759

    Purpose

    sgRNA2 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

    Depositor

    Glenn Yiu

    Article

    Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

    Insert

    gRNA2 targeting VEGF-A (VEGFA Mouse, M. mulatta (rhesus macaque), Human)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCJH009_proD_Cas12c-sgRNA-GFP_CAM

    Plasmid

    #183070

    Purpose

    Plasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c single guide RNA targeting GFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. gRNA-hIRF-1 #12/pSIR-hCD2

    Plasmid

    #135392

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    gRNA_hIRF1 promoter #12
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCB2-sgRNA

    Plasmid

    #129463

    Purpose

    Template plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    pgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid

    #73796

    Purpose

    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

    Depositor

    Ronald Davis

    Article

    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

    Inserts

    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1, pRPR1(TetO), and pTef1

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122090

    Purpose

    U6 driven SaCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPEgRNA

    Plasmid

    #172716

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pnsgRNA

    Plasmid

    #172717

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    J23119-gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge