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Plasmid#58727Purposeexpression of mouse Alpha2 delta-3 in mammalian cellsDepositorInsertalpha2 delta-3 (Cacna2d3 Mouse)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-NOG(AtUBI10)
Plasmid#202016PurposeT-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.DepositorInsertsdCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1
UseTagsGB1 and sfGFPExpressionPlantMutationPromoterAtUBI10Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))
Plasmid#164262PurposeVector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter.DepositorInsertCodon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTP170 (pBAD33_CRISPR(Tn6900)-entry)
Plasmid#164263PurposeEntry vector for cloning user-defined spacers in the Tn6900 CRISPR array.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterArabinoseAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-RanGAP
Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
UseTagsAviExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mouse Alpha2 delta-3 HA pMT2
Plasmid#58728Purposeexpression of HA tagged mouse Alpha2 delta-3 in mamalian cellsDepositorInsertalpha2 delta-3 (Cacna2d3 Mouse)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Rat Alpha2 delta-1 pMT2
Plasmid#58726Purposeexpression of rat alpha2 delta-1 in mammalian cellsDepositorInsertalpha2 delta-1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
cacnb1b (rat) in pMT2 vector
Plasmid#107423PurposeExpresses Calcium channel beta1b auxillary subunit in mammalian cells.DepositorInsertcalcium channel beta1b auxillary subunit
UseTagsExpressionMammalianMutationPromoterAd MLPAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-NLS-BirA-2A-mCherry_Ras
Plasmid#80067PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E K91N
Plasmid#110121PurposeExpresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activityDepositorInsertHel25E (Hel25E Fly)
UseTagsFlagExpressionInsectMutationK91N mutationPromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E D193E
Plasmid#110120PurposeExpresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinityDepositorInsertHel25E (Hel25E Fly)
UseTagsFlagExpressionInsectMutationD193E mutationPromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-cytoBirA-2a-mCherry
Plasmid#79885PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-cytoBirA-2A-mCherry_Ras
Plasmid#80058PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-cytoBirA-2A-mCherry_Ras
Plasmid#80060PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterMyl7 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+eGFP-bromo5(N140A)
Plasmid#157806PurposeExpression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutationDepositorInserteGFP-bromo5(N140A)
UseTagsHis6 and MBPExpressionBacterialMutationN140APromoterpTACAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-flk1-cytoBirA-2A-mCherry_Ras
Plasmid#80056PurposeFlk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterflk1Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-zic2a-cytoBirA-2A-mCherry_Ras
Plasmid#80064PurposeZic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterZic2a enhancer driving c-fos promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-HGP-v3-Nb127D01-hIgG
Plasmid#171564PurposeExpresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cellsDepositorInsertNb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
UseTagsBiP signal peptide and hIgG1-hinge-N297A-TEV site…ExpressionInsectMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-HGP-v3-NbVHH05-hIgG
Plasmid#171565PurposeExpresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cellsDepositorInsertNbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)
UseTagsBiP signal peptide and hIgG1-hinge-N297A-TEV site…ExpressionInsectMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-LANA-H1.4CTD
Plasmid#157802PurposeExpression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion proteinDepositorInsertmEGFP-LANA-H1.4CTD (H1-4 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only