We narrowed to 9,747 results for: ada

1. pEGFP-UBE4B

   Plasmid

   #113499

   Purpose

   Expression of human UBE4B with N-terminal GFP tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   UBE4B (UBE4B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi_RP_2XMTS-MicroID2-FLAG

   Plasmid

   #188524

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   2XMTS-MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-U6-crRNA-empty

   Plasmid

   #134921

   Purpose

   Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   crRNA for CRISPR-Cas3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK425 (GAL-OsTIR1(F74A))

   Plasmid

   #140657

   Purpose

   GAL-OsTIR1(F74A)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74A)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xHis-SNAP-PI(3,5)P2 Probe

   Plasmid

   #211512

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   SnxA-2xPX
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-FLAG-Claspin

   Plasmid

   #12659

   Depositor

   Michele Pagano

   Article

   Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

   Insert

   Claspin (CLSPN Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2007

   Availability

   Academic Institutions and Nonprofits only

7. 6xHis-SNAP-PI(3,4)P2 Probe

   Plasmid

   #211511

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   TAPP1 cPHx3
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6xHis-SNAP-PI(3,4,5)P3 Probe

   Plasmid

   #211514

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   BTK1-PH
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDIV270

   Plasmid

   #226181

   Purpose

   Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF1p (Komagataella phaffii)

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP

    Plasmid

    #40640

    Depositor

    Brenda Lilly

    Article

    Zhao et al J Biol Chem. 2012 May 11;287(20):16111-2

    Insert

    NICD3 (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-AAVS1-PGK-Blast

    Plasmid

    #227270

    Purpose

    Empty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG/ATP10D(E215Q)-HA

    Plasmid

    #209248

    Purpose

    Mammalian expression of ATP10D mutant E215Q

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10D (ATP10D Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E215Q

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-PITCh-H2BC11

    Plasmid

    #207755

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP.N1-HDAC6.DC (catalytic deficient)

    Plasmid

    #36189

    Depositor

    Tso-Pang Yao

    Article

    Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

    Insert

    HDAC6.DC (HDAC6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    H216/611A

    Promoter

    CMV

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

    Plasmid

    #159807

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-AviTag-DogCatcher-MBP

    Plasmid

    #171928

    Purpose

    Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    AviTag-DogCatcher-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    DogCatcher added to N-terminus of MBP via a GSGES…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK419 (GAL-OsTIR1(F74G))

    Plasmid

    #140656

    Purpose

    GAL-OsTIR1(F74G)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    GAL-OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF5B-FRT-cilia-APEX

    Plasmid

    #73186

    Purpose

    Expresses cilia-APEX in mammalian cells

    Depositor

    Maxence Nachury

    Article

    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

    Insert

    cilia-APEX (Nphp3 Glycine max, Mouse)
    

    Use

    Tags

    internal GFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-IP HA-Parkin

    Plasmid

    #38248

    Depositor

    Noboru Mizushima

    Article

    Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

    Insert

    Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-AAVS1-PGK-Puro

    Plasmid

    #227269

    Purpose

    Empty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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