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We narrowed to 40,142 results for: ina

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Showing: 3581 - 3600 of 40142 results

  4. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Publication
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  12. GFP-α-cateninA+

    Plasmid
    #229697
    Purpose
    Transient expression of GFP-alpha-cateninA+ in mammalian cells; enhanced actin binding
    Depositor
    Alpha Yap
    Publication
    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    amino acids 670-673, RAIM--> GSGS
    Promoter
    CMV promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229697-plasmid-map-sequence-id-458274

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  14. pACYC Destination

    Plasmid
    #207675
    Purpose
    Trio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).
    Depositor
    Ramon Gonzalez
    Publication
    Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    CymRC, PhlF, VanCC
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    207675-plasmid-map-sequence-id-421910

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  15. pBridge-tyrosinase.σ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Publication
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-1 σ1A
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4…
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197381-plasmid-map-sequence-id-388878

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Showing: 3581 - 3600 of 40142 results