We narrowed to 14,938 results for: pET

1. pET-29b(+)_His-CBD-3C-EmPNGaseF

   Plasmid

   #218193

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF

   Depositor

   Florence Pojer

   Insert

   PNGaseF
   

   Use

   Tags

   tufA-His-CBD-3C

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 41-354

   Promoter

   T7

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-21a(+)/MBP-His-PINK1

   Plasmid

   #38005

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   PINK1 (PINK1 Human)
   

   Use

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pET22b-PelB-6H-Ag43-160N-sfGFP

   Plasmid

   #172886

   Purpose

   Display sfGFP protein on E. coli cell surface

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   SfGFP
   Ag43, partial (flu )
   

   Use

   Tags

   HHHHHH and TEV recognition site (ENLYFQG)

   Expression

   Bacterial

   Mutation

   Deleted 1-160 (N terminal)

   Promoter

   T7 Promoter

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pet28a-His6-Halo-TEV-Nrf2

   Plasmid

   #62455

   Purpose

   Halo-His6 tagged Nrf2 for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Nrf2 (NFE2L2 Human)
   

   Use

   Tags

   His6-Halo-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-28a-YTH domain of YTHDF2

   Plasmid

   #228776

   Purpose

   Protein expression plasmid for YTH domain of YTHDF2 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   6*His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a(+)-T7-OmpA-MNaseA-6xHis

   Plasmid

   #214808

   Purpose

   Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

   Depositor

   Peter Sarin

   Article

   Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

   Insert

   OmpA-Micrococcal nuclease A
   

   Use

   Tags

   6xhis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-16b-10His-TEV-MCES_VACCA_D1_D12

   Plasmid

   #203671

   Purpose

   The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

   Depositor

   Stephen Cusack

   Article

   Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

   Insert

   MCES_VACCA_D1_D12
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30-HPF1-His-Sumo-Flag

   Plasmid

   #111577

   Purpose

   Expresses full length HPF1 in E. coli

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   HPF1 (HPF1 Human)
   

   Use

   Tags

   6xHis-Sumo-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET51b-His-TEV-SNAP-tag_fast

   Plasmid

   #167271

   Purpose

   Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SNAPf-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   E30R

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-28a+GFP-CAMSAP3 CKK

    Plasmid

    #59033

    Purpose

    expresses CKK domain of CAMSAP3 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP3 CKK domain (CAMSAP3 Mouse)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pET-22b(+) pCopA RBS30 mscarlet

    Plasmid

    #226370

    Purpose

    Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    mScarlet
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Promoter

    copA promoter (pCopA)

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid

    #225139

    Purpose

    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

    Depositor

    Urartu Seker

    Article

    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.

    Insert

    Ag43 protein fused to silaffin-R5 and sfGFP
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

    Plasmid

    #117910

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R44A on eDHFR

    Promoter

    T7

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-10XHis-GST-HRV-dL5

    Plasmid

    #73214

    Purpose

    Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

    Insert

    EGFR (EGFR Human, Schistosoma japonicum)
    

    Use

    Tags

    10XHis-GST-HRV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET51b-His-TEV-SNAP-tag

    Plasmid

    #167269

    Purpose

    Overexpression of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid

    #177334

    Purpose

    bacterial expression of active SARS-CoV-2 3C-like proteinase

    Depositor

    Christopher Overall

    Article

    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Use

    Tags

    Factor Xa site-3xFlag-Myc-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available since

    Nov. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pPS22 (pET23a-Cas10/Csm/csm2H6N)

    Plasmid

    #70038

    Purpose

    Bacterial expression of the S. epidermidis Cas10·Csm complex

    Depositor

    Luciano Marraffini

    Article

    Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

    Insert

    Cas10/Csm complex
    

    Use

    Tags

    6xHIS on N terminus of Csm2

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETDuet-1 Hepatitis C Virus NS3/NS4A

    Plasmid

    #61696

    Purpose

    inducible dual expression of NS3 protease along with its cofactor NS4A

    Depositor

    Michael Johnson

    Article

    Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

    Inserts

    NS4A
    NS3
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21a(+)-HIV RT-6xHis-RBS-prot

    Plasmid

    #159149

    Purpose

    expresses His-tagged HIV reverse transcriptase and untagged HIV protease

    Depositor

    Andrea Pauli

    Article

    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

    Inserts

    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
    Human immunodeficiency virus 1 (HIV-1) protease
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    HIV-1 group M/subtype B – BH10 strain

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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