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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,041 results for: pET

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Showing: 3581 - 3600 of 10041 results

  5. pTO149_RFP_CauNEO

    Plasmid
    #177277
    Purpose
    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.
    Depositor
    Teresa O'Meara
    Publication
    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:
    Insert
    RFP, CauNEO
    Tags
    ENO1-RFP
    Expression
    Bacterial
    Mutation
    All CUG Leucine codons were replaced with the syn…
    Promoter
    AgTEF2
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    177277-plasmid-map-sequence-id-347241

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  6. pET-28b-T7-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1897)

    Plasmid
    #114074
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R, K548V and N552R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114074-plasmid-map-sequence-id-220510

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  7. GEF-H1 FLARE212.pa

    Plasmid
    #134930
    Purpose
    GEF-H1 biosensor that does not have GEF activity.
    Depositor
    Klaus Hahn
    Publication
    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
    Tags
    None.
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and T7
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    134930-plasmid-map-sequence-id-271271

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  8. pcDNA3-PM-CaNAR2

    Plasmid
    #64730
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-CaNAR2
    Tags
    Cerulean3, N-terminal targeting sequence from Lyn…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64730-plasmid-map-sequence-id-109348

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  14. pET28a-6xHis-SURF6

    Plasmid
    #255296
    Purpose
    SURF6 (1-361) with an N-terminal 6X poly-histidine tag and a TEV recognition site sub-cloned into pET28 plasmids with Kanamycin bacterial resistance, were used for protein expression.
    Depositor
    Richard Kriwacki
    Publication
    Dogra et al bioRxiv 2025.03.01.640913
    Insert
    SURF6 (SURF6 Human)
    Tags
    6x-His
    Expression
    Bacterial
    Promoter
    T7
    Available since
    May 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    255296-plasmid-map-sequence-id-509016

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Showing: 3581 - 3600 of 10041 results