We narrowed to 4,339 results for: Pet2;

1. pET28a SUMO Nup53xl 162-267

   Plasmid

   #86002

   Purpose

   bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53

   Depositor

   Wolfram Antonin

   Type

   Empty backbone

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-FgßF3-ddFLN4-HIS-ybbr

   Plasmid

   #101743

   Purpose

   mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   FgßF3 peptide, ddFLN4 fingerprint (FGB Dictyostelium discoideum, Human)
   

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4, added 9F in FgßF3

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. delNC C-His Ana GvpC in pET28a

   Plasmid

   #85736

   Purpose

   Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (truncated and his-tagged)
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Deletion of N and C terminal regions, addition of…

   Promoter

   T7

   Available Since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-ybbR-HIS-CBM-CohI

   Plasmid

   #58709

   Purpose

   Probing molecule to perform single molecule force spectroscopy experiments

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   CBM-CohI
   

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   changed Alanine 2 to Cysteine in CBM domain

   Promoter

   T7

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-NmCas9-Flag D16A+H588A

   Plasmid

   #109033

   Purpose

   D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Tags

   Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-Hook3 I136A, I139A, M142A

   Plasmid

   #74617

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
   

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   D102A, E108A

   Promoter

   T7

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b(+)_S. cerevisiae Hbs1(166-611)

   Plasmid

   #39338

   Depositor

   SeWon Suh

   Insert

   Elongation factor 1 alpha-like protein
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pET28a-ybbR-HIS-10FNIII(x4)-DocI

   Plasmid

   #58712

   Purpose

   Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   10FNIII(x4)-DocI
   

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a(+)v2_TwinStrep-SUMO-FLP-8xHis

   Plasmid

   #246980

   Purpose

   Expresses E. coli codon-optimized TwinStrep-SUMO-tagged FLP recombinase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.

   Depositor

   Amro Hamdoun

   Insert

   FLP Recombinase
   

   Use

   Synthetic Biology

   Tags

   8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-His6-GFP-UROD-N367A

    Plasmid

    #236742

    Purpose

    Protein overexpression of eGFP-human uroporphyrinogen decarboxylase N367A

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    eGFP-Uroporphyrinogen decarboxylase human N367A (UROD Synthetic, Human)
    

    Tags

    His6 and eGFP

    Expression

    Bacterial

    Mutation

    N367A

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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