We narrowed to 2,346 results for: puc19

1. pAT15544_nABE-SuperFi-Cas9

   Plasmid

   #184374

   Purpose

   Mammalian expression of SuperFi-Cas9 ABE7 base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   nABE-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   FLAG, SV40 NLS, and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

   Promoter

   EF-1α core promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEasyG2_zeo

   Plasmid

   #184913

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEasyG2_nat

   Plasmid

   #184914

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEasyG3_zeo

   Plasmid

   #184917

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. ATP1A1_G8_MTOR_Nick_Exon-53_Dual_sgRNA

   Plasmid

   #178107

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G8 + MTOR nick exon-53 sgRNAs
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJBL729

   Plasmid

   #140399

   Purpose

   Expresses CadC-regulated 3WJdB RNA aptamer driven by T7 promoter with the cadO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-cadO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJBL718

   Plasmid

   #140388

   Purpose

   Expresses MobR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mobO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-mobO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTW324

   Plasmid

   #115955

   Purpose

   Destination Vector

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl1 P1 End
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTW064

   Plasmid

   #115951

   Purpose

   Destination Vector

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl0 ORF Dest
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

    Plasmid

    #173214

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
    

    Use

    CRISPR; Prime editing (pe3)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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