We narrowed to 9,668 results for: BLI

1. Ec holB-pET(11a)(ACH-)

   Plasmid

   #237234

   Purpose

   over-express E. coli holB (Pol III delta prime subunit)

   Depositor

   Michael O'Donnell

   Insert

   holB (delta prime) (holB E. coli)
   

   Expression

   Bacterial

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG426-GAL-3xFLAG-PolyS

   Plasmid

   #181728

   Purpose

   Galactose inducible expression of 3xFLAG-PolyS

   Depositor

   Alejandro Chavez

   Insert

   3xFLAG-PolyS
   

   Tags

   3xFLAG

   Expression

   Yeast

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG426-GAL-3xFLAG-PolyA

   Plasmid

   #181726

   Purpose

   Galactose inducible expression of 3xFLAG-PolyA

   Depositor

   Alejandro Chavez

   Insert

   3xFLAG-PolyA
   

   Tags

   3xFLAG

   Expression

   Yeast

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG426-GAL-3xFLAG-PolyL

   Plasmid

   #181727

   Purpose

   Galactose inducible expression of 3xFLAG-PolyL

   Depositor

   Alejandro Chavez

   Insert

   3xFLAG-PolyL
   

   Tags

   3xFLAG

   Expression

   Yeast

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG426-GAL-3xFLAG-PolyC

   Plasmid

   #181729

   Purpose

   Galactose inducible expression of 3xFLAG-PolyC

   Depositor

   Alejandro Chavez

   Insert

   3xFLAG-PolyC
   

   Tags

   3xFLAG

   Expression

   Yeast

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. EP003: CAG::GEM7-HaloTag

   Plasmid

   #209303

   Purpose

   Expression plasmid for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice via in utero electroporation

   Depositor

   Adam Cohen

   Insert

   GEM7
   

   Use

   AAV

   Tags

   HaloTag

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15bHis6-pG-MNase

   Plasmid

   #124886

   Purpose

   To transform BL21-Gold(DE3) and be induced by IPTG for His-proteinG-MNase fusion protein expression.

   Depositor

   Keji Zhao

   Insert

   protein G-MNase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-hSyncytin-1(deltaFP)

   Plasmid

   #182443

   Purpose

   hSyncytin-1, fusion peptide deletion(320-340)

   Depositor

   Masaharu Somiya

   Insert

   ERVW-1 (ERVW-1 Human)
   

   Expression

   Mammalian

   Mutation

   no fusion peptide (320-340)

   Promoter

   CMV-F

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hSyncytin-1(C186A)

   Plasmid

   #182450

   Purpose

   hSyncytin-1, fusion deficit and dominant negative mutant (C186A)

   Depositor

   Masaharu Somiya

   Insert

   ERVW-1 (ERVW-1 Human)
   

   Expression

   Mammalian

   Mutation

   C186A

   Promoter

   CMV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG Retro EGFP Puro

    Plasmid

    #234463

    Purpose

    Control retroviral vector expressing Green Fluorescent Protein. Puromycin selection.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Green Fluorescent Protein
    

    Use

    Retroviral

    Available Since

    March 21, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAG Retro EGFP Hygro

    Plasmid

    #234462

    Purpose

    Control retroviral vector expressing Green Fluorescent Protein. Hygromycin selection.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Green Fluorescent Protein
    

    Use

    Retroviral

    Available Since

    March 21, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCWU50

    Plasmid

    #232318

    Purpose

    A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch

    Depositor

    Chenggang Wu

    Insert

    Pfdx-E
    

    Expression

    Bacterial

    Promoter

    fdx

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. 1203AE-CMV-Cas7.1-7.2-H43A-mutant

    Plasmid

    #221449

    Purpose

    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, H to A mutation at amino acid 43 in Cas7.1

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1203AF-CMV-Cas7.1-7.2-Y55A-mutant

    Plasmid

    #221450

    Purpose

    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, Y to A mutation at amino acid 55 in Cas7.1

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1203AG-CMV-Cas7.1-7.2-N152A-mutant

    Plasmid

    #221451

    Purpose

    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, N to A mutation at amino acid 152 in Cas7.1

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-N-TEV-Twin-Strep

    Plasmid

    #222014

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli

    Depositor

    Chiaki Murakami

    Type

    Empty backbone

    Tags

    TEV cleavable site (ENLYFQG) and Twin-strep-tag

    Expression

    Bacterial

    Promoter

    lac promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpec5'

    Plasmid

    #228549

    Purpose

    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 5' region of aadA
    

    Use

    CRISPR

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpec3'

    Plasmid

    #228548

    Purpose

    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUSE-URA3-Sec7-mEYFP

    Plasmid

    #218968

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with mEYFP

    Depositor

    Benjamin Glick

    Insert

    SEC7-mEYFP (SEC7 Budding Yeast)
    

    Use

    Gene replacement

    Tags

    mEYFP

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUSE-URA-Sec7-mGold

    Plasmid

    #218967

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with mGold

    Depositor

    Benjamin Glick

    Insert

    SEC7-mGold (SEC7 Budding Yeast)
    

    Use

    Gene replacement vector for yeast

    Tags

    mGold

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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