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miniSOG-Vimentin-7

Plasmid

#57779

Purpose

Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Vimentin (VIM Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
xfz2N/pCS2+

Plasmid

#16329

Depositor

Peter Klein

Publication

Deardorff et al Mech Dev. 1999 Sep . 87(1-2):229-33.

Insert

frizzled-2 (fzd2 Frog)

Use

Vertebrate expression

Mutation

Extracellular ligand binding domain of Xenopus fr…

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pFUW H2B-mEmerald-P2A-PuroR

Plasmid

#239553

Purpose

Expression and nuclear localization of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Publication

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tbg-CES2A-ΔC

Plasmid

#200558

Purpose

Secreted mouse CES2A driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Publication

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)

Use

AAV

Tags

Flag

Promoter

TBG promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_GFP10-14-SEPT7

Plasmid

#180341

Purpose

mammalian expression of human SEPT7 fused to GFP10

Depositor

Manos Mavrakis

Publication

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

GFP10

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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XIAP (pBS0852)

Plasmid

#185282

Purpose

For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

XIAP (XIAP Human)

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGIPz-PD-L1 3SA

Plasmid

#121490

Purpose

Expression of human PD-L1 3SA with PD-L1 shRNA

Depositor

Mien-Chie Hung

Publication

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Inserts

PD-L1 3SA
humun PD-L1 shRNA

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

S176A, T180A, S184A

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_MAPK3_Pkinase

Plasmid

#110023

Purpose

Protein expression and purification of MAPK3_Pkinase

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

MAPK3_Pkinase (MAPK3 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_EEF1A2_GTP-EFTU

Plasmid

#109862

Purpose

Protein expression and purification of EEF1A2_GTP-EFTU

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EEF1A2_GTP-EFTU (EEF1A2 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPB CAG Gfi1:FLAG-IRES-EGFP

Plasmid

#153945

Purpose

piggyBac transposon vector with CAG promoter expressing FLAG-tagged Gfi1 and EGFP

Depositor

Luca Tiberi

Publication

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Gfi1 (Gfi1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GABARα1N-HA

Plasmid

#128110

Purpose

Express GABARα1 N terminal domain

Depositor

Huda Zoghbi

Publication

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Gabra1 (Gabra1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2281

Plasmid

#111902

Purpose

ssAAV genome with Ple346 (NEFM MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple346 (NEFM Human)

Use

AAV

Promoter

Ple346 (NEFM MiniPromoter)

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCZGY2162

Plasmid

#110136

Purpose

Epidermal Mitochondrial-tagged Florescent Calcium Sensor

Depositor

Andrew Chisholm

Publication

Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

Insert

mito::GCaMP3

Tags

mito::GCaMP3

Expression

Worm

Promoter

Pcol-19

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCRII-Sema4d-ISH-2kb

Plasmid

#68032

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Publication

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4D (Sema4d Mouse)

Use

Mrna in situ hybridization probe

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pAT2732-(PB-4_EF1α-Hygro_2A_rtTA3 [HA-BFP-KRAB-PUFa])

Plasmid

#251107

Purpose

Expression of KRAB-PUFa component of Casilio-i system

Depositor

Chia-Lin Wei

Publication

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

BFP-KRAB-PUFa

Tags

HA

Expression

Mammalian

Mutation

N/A

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FlpX-mKate2.0-N2cG

Plasmid

#234711

Purpose

pAAV transfer plasmid with CAG promoter driving Flp-dependent expression of mKate2.0 and N2c G protein separated by a T2A linker

Depositor

David Ng

Publication

Mendelsohn et al Cell Rep. 2025 Apr 22;44(4):115454. doi:

Insert

mKate2.0 T2A N2cG

Use

AAV

Tags

T2A

Expression

Mammalian

Promoter

CAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pStreptagII 3C SMO

Plasmid

#234995

Purpose

Expression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptor

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Publication

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

SMO (SMO Human)

Tags

Streptag-II, HRV 3C site

Expression

Mammalian

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF-MjCouRS

Plasmid

#229066

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Publication

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

Methanococcus jannaschii tRNA synthetase

Expression

Bacterial

Promoter

T7

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227892

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Publication

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

EEA1 homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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mActA-mSspB-mCherry

Plasmid

#210294

Purpose

ActuAtor for iLID optogenetics

Depositor

Takanari Inoue

Publication

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

mActA-,SspB-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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