We narrowed to 5,897 results for: ARL

1. pXPR_070

   Plasmid

   #164265

   Purpose

   Lentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

   Insert

   sgRNA cassette
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-Cdt1ΔPIP-mCherry-Puro

   Plasmid

   #193765

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1ΔPIP (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   Cdt1ΔPIP-mCherry (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   PIP degron removed (a.a.1-19 removed)

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Venus-cpVenus-FLARE-Cameleon

   Plasmid

   #123350

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-Cameleon
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Venus-cpVenus-FLARE-ICUE

   Plasmid

   #123355

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-ICUE
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Venus-cpVenus-FLARE-AKAR

   Plasmid

   #123329

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-AKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

   Plasmid

   #123330

   Purpose

   Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-AKAR(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6/V5-DEST-HSF1

   Plasmid

   #31210

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   HSF1 (HSF1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pEGFP C1-Eps8 WT

   Plasmid

   #74950

   Purpose

   mammalian expression of Eps8 fused to GFP

   Depositor

   Giorgio Scita

   Article

   Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

   Insert

   Eps8 (Eps8 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

9. AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)

   Plasmid

   #165456

   Purpose

   Inducible ZEB2 expression from AAVS1 locus

   Depositor

   Madeline Lancaster

   Article

   Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

   Insert

   ZEB2 (ZEB2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE-hygro p53 DD

    Plasmid

    #9058

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    p53 DD (Trp53 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    DD. Dominant negative mutant carries deletion of…

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX209-neo-HA-Ub-T2A-Tet3G

    Plasmid

    #202436

    Purpose

    Bicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and Tet-On 3G transactivator (Tet3G)

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    Ubiquitin
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti6/V5-DEST-HMGB1

    Plasmid

    #31208

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pJM185A

    Plasmid

    #218126

    Purpose

    Hybrid circuit displaying hsCRBN bait and driving expression of luxAB

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    CRBN (CRBN Human)
    luxAB
    

    Use

    Tags

    434cI_RR69

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-mCherry-hCdt1(1-100)ΔCy

    Plasmid

    #193759

    Purpose

    Expresses N-CRL4Cdt2 reporter(mCherry-hCdt1(1-100)ΔCy)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    mCherry-hCdt1(1-100)ΔCy (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

    Promoter

    EF1a

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pST_140_LVL2 cam

    Plasmid

    #179334

    Purpose

    NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tet-O-FUW-EGFP-P2A-mCherry

    Plasmid

    #203868

    Purpose

    Tet-inducible lentiviral plasmid expressing EGFP-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. MOM603

    Plasmid

    #133242

    Purpose

    expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

    Depositor

    Shinsuke Niwa

    Article

    Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

    Insert

    kif1a (KIF1A Human)
    

    Use

    Tags

    StrepII tag and mScarlet

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6/V5-DEST-MCM7

    Plasmid

    #31212

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    MCM7 (MCM7 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

19. DRH323: pAAV_hSyn-QuasAr2

    Plasmid

    #107705

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    QuasAr2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICSL11060

    Plasmid

    #68264

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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