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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 3621 - 3640 of 18824 results

  1. pCM42

    Plasmid
    #261129
    Purpose
    bJJUN::VN173 and mTagBFP expression under myo-3 promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    myo-3p::AID::nls::mTagbfp2::2a::AID::bJUN::VN173::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Mutation
    bJUN
    Promoter
    myo-3
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    261129-plasmid-map-sequence-id-520973

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  2. pCM41

    Plasmid
    #261128
    Purpose
    bFOSdeltaZip::VC155 and mCherry expression under myo-3 promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    myo-3p::AID::nls::mcherry::2a::AID::bFOSdeltaZIP::VC155::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Mutation
    bFOSdeltaZIP
    Promoter
    myo-3
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    261128-plasmid-map-sequence-id-520969

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  3. pCM37

    Plasmid
    #261124
    Purpose
    bJUN::VN173 and mTagBFP expression under rgef-1 promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    rgef-1p::AID::nls::mTagbfp2::2a::AID::bJUN::VN173::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Mutation
    bJUN
    Promoter
    myo-3
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    261124-plasmid-map-sequence-id-520104

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  4. pCM35

    Plasmid
    #261122
    Purpose
    bFOS::VC155 and mCherry expression under rgef-1 promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    rgef-1p::AID::nls::mcherry::2a::AID::bFOS::VC155::tbb-2 3'in pLF3FShC
    Expression
    Worm
    Mutation
    bFOS
    Promoter
    rgef-1
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    261122-plasmid-map-sequence-id-520094

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  5. L2_2xNOP1gRNA-Cas9-CsA

    Plasmid
    #136140
    Purpose
    Plasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygR
    Depositor
    Jim Haseloff
    Publication
    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102
    Insert
    p5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI/ SapI domesticated
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    136140-plasmid-map-sequence-id-275306

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  8. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid
    #138143
    Purpose
    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
    Depositor
    Neville Sanjana
    Publication
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PguCas13b
    Use
    Lentiviral
    Tags
    NLS
    Available since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138143-plasmid-map-sequence-id-275400

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  15. pcDNA3-His:CRY2:nls:VPR

    Plasmid
    #135989
    Purpose
    Expresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CRY2 domain fused to the VPR activation domain
    CRY2 domain fused to the VPR activation domain
    Tags
    His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135989-plasmid-map-sequence-id-269558

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  19. pAcCas12nHS_ABE_D3_empty

    Plasmid
    #203809
    Purpose
    Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).
    Depositor
    Quanjiang Ji
    Publication
    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE_D3
    Use
    CRISPR
    Promoter
    CMV
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203809-plasmid-map-sequence-id-405073

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Showing: 3621 - 3640 of 18824 results