We narrowed to 17,428 results for: She

1. pEF-GalNAcT2-mGold

   Plasmid

   #218964

   Purpose

   Expresses full length GalNAcT2-mGold in mammalian cells

   Depositor

   Benjamin Glick

   Insert

   GalNAcT2-mGold (GALNT2 Human)
   

   Use

   Tags

   mGold

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEF-GalNAcT2-msYFP2

   Plasmid

   #218963

   Purpose

   Expresses full length GalNAcT2-msYFP2 in mammalian cells

   Depositor

   Benjamin Glick

   Insert

   GalNAcT2-msYFP2 (GALNT2 Human)
   

   Use

   Tags

   msYFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEF-GalNAcT2-msGFP2

   Plasmid

   #218965

   Purpose

   Expresses full length GalNAcT2-msGFP2 mammalian cells

   Depositor

   Benjamin Glick

   Insert

   GalNAcT2-msGFP2 (GALNT2 Human)
   

   Use

   Tags

   msGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA3.SLC17A1-flag

   Plasmid

   #224418

   Purpose

   Express SLC17A1 in mammalian cells

   Depositor

   Jonathan Long

   Insert

   Solute carrier family 17 (sodium phosphate), member 1 (SLC17A1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lentiGuideB3GNT5-neo

   Plasmid

   #220867

   Purpose

   encodes CRISPR sgRNA targeting human B3GNT5

   Depositor

   Jennifer Kohler

   Insert

   B3GNT5 (B3GNT5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. tRECs/3xUAS-FP attB

   Plasmid

   #220330

   Purpose

   encodes three Fluorescent Protein FLP-OUT cassettes responsive to three different recombinases (mFLP5, B3 and KD)

   Depositor

   Erdem Bangi

   Inserts

   B3RT-STOP-B3RT-TagBFP-3xHA
   KDRT-STOP-KDRT-GFP-HSV
   FRTm71-STOP-FRTm71-mCherry
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAS

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-M566T

   Plasmid

   #218832

   Purpose

   Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant M566T for BioID experiments.

   Depositor

   Travis Stracker

   Insert

   TLK1 M566T (TLK1 Human)
   

   Use

   Tags

   FLAG-BirA*

   Expression

   Mammalian

   Mutation

   M566T

   Promoter

   CMV

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-Q479E

   Plasmid

   #218833

   Purpose

   Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant Q479E for BioID experiments.

   Depositor

   Travis Stracker

   Insert

   TLK1 Q479E (TLK1 Human)
   

   Use

   Tags

   FLAG-BirA*

   Expression

   Mammalian

   Mutation

   Q479E

   Promoter

   CMV

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PE-SUMO CRYGA

   Plasmid

   #217672

   Purpose

   For expression of SUMO tagged human gamma A crystallin

   Depositor

   Larry David

   Insert

   CRYGA (CRYGA Human)
   

   Use

   Tags

   6XHis and Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn-frt-DIO-hM3D(Gq)-mCherry

    Plasmid

    #217397

    Purpose

    Expresses the hM3D(Gq) excitatory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoter

    Depositor

    Oscar Marin

    Insert

    hM3D(Gq)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-frt-DIO-hM4D(Gi)-mCherry

    Plasmid

    #217398

    Purpose

    Expresses the hM4D(Gi) inhibitory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoter

    Depositor

    Oscar Marin

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRIPZ-NUDT18-C-FLAG-Puro

    Plasmid

    #195181

    Purpose

    Lentiviral vector for DOX-inducible NUDT18 gene expression with C-terminal FLAG tag. Gateway expression system. Selection by puromycin.

    Depositor

    Stephanie Roessler

    Insert

    NUDT18 (NUDT18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA

    Plasmid

    #191528

    Purpose

    Vector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabain

    Depositor

    Yannick Doyon

    Insert

    SLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDest_U6_A2_CG2_T1snp_gRNA

    Plasmid

    #213789

    Purpose

    pDEL114; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I (SNP) sgRNA

    Depositor

    William Talbot

    Insert

    nrg1 type I SNP sgRNA (nrg1 Zebrafish)
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Cas9-T2A-H2B mCitirine

    Plasmid

    #210471

    Purpose

    Coexpression of Cas9 and H2B mCitrine

    Depositor

    Amro Hamdoun

    Insert

    Cas9-T2A-H2B mCitirine
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mCitrine on C terminal of H2B

    Expression

    Mammalian

    Mutation

    Cas9 contains 3xFLAG tag and nuclear localization…

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAttB-Tub-HA-Wnk

    Plasmid

    #203968

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (most similar to RO variant) (Wnk Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAttB-Tub-HA-WnkDeltaNT

    Plasmid

    #203969

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    Nterm deletion

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAttB-Tub-HA-WnkDeltaAI

    Plasmid

    #203970

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    deletion of autoinhibitory domain

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAttB-Tub-HA-WnkDeltaMID

    Plasmid

    #203971

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    deletion of middle part

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAttB-Tub-HA-WnkDeltaCC

    Plasmid

    #203972

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    deletion of coiled coil domains 1 and 2

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAttB-Tub-HA-WnkDeltaCC1

    Plasmid

    #203973

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    deletion of coiled coil domain 1

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAttB-Tub-HA-WnkDeltaCC2

    Plasmid

    #203974

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    deletion of coiled coil domain 2

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAttB-Tub-HA-WnkDeltaCT

    Plasmid

    #203975

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    C-terminal deletion after RFxV motif

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 3xFlag-CEP83

    Plasmid

    #200442

    Purpose

    Expresses 3xFlag-tagged mouse CEP83 in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Insert

    CEP83 (CCDC41 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSBtet-Bla/mCardinal-TurboID-GAI

    Plasmid

    #198218

    Purpose

    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with mCardinal, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling

    Depositor

    Eric Davis

    Insert

    mCardinal
    

    Use

    Transposon

    Tags

    3’ part of TurboID, starting with G257 and amino …

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGB-05-08-Vps15

    Plasmid

    #187990

    Purpose

    Plasmid for the protein expression of Vps15 in a bacterial and insect expression system. Internal_Reference: SMC1324

    Depositor

    Sascha Martens

    Insert

    Vps15 (PIK3R4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

    Plasmid

    #166422

    Purpose

    Express pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Insert

    Plasmodium falciparum PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCW57.1-RFP_mmu-miR-292b-3p_sponge

    Plasmid

    #132623

    Purpose

    Expresses a TurboRFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

    Depositor

    Constance Ciaudo

    Insert

    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Akaluc VLP

    Plasmid

    #182212

    Purpose

    YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter).

    Depositor

    Claudia Vickers

    Inserts

    VP2C-Akaluc
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology; Tem imaging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MDKA-c009

    Plasmid

    #175463

    Purpose

    Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143

    Depositor

    Opher Gileadi

    Insert

    MDK (MDK Human)
    

    Use

    Tags

    His6 and PTPRS (Gg) SP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. EZRA-c004

    Plasmid

    #175461

    Purpose

    Protein expression in bacterial cells. FERM domain, M1-Q322.

    Depositor

    Opher Gileadi

    Insert

    EZR (EZR Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. NF2A-c001

    Plasmid

    #175472

    Purpose

    Protein expression in bacterial cells. FERM domain, M1-R338.

    Depositor

    Opher Gileadi

    Insert

    NF2 (NF2 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. EPB41L3A-c001

    Plasmid

    #175459

    Purpose

    Protein expression in bacterial cells. FERM domain, M1-L390.

    Depositor

    Opher Gileadi

    Insert

    EPB41L3 (EPB41L3 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMta2-EosHalo-TAFiP

    Plasmid

    #140959

    Purpose

    Targeting construct to make Mta2-Eos-Halo-Tev-Avi-3xFlag fusion

    Depositor

    Brian Hendrich

    Insert

    metastasis-associated gene family, member 2 (Mta2 Synthetic, Mouse)
    

    Use

    Mouse Targeting

    Tags

    3xFLAG, Avi, Eos, Halo, and TEV

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. XLone-BSD NFIL3 T2A Puro

    Plasmid

    #140028

    Purpose

    Tunable and temporal expression control of NFIL3 and puro resistant gene

    Depositor

    Xiaoping Bao

    Insert

    NFIL3 (NFIL3 Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    puromycin

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3. 3xFlag:PP4-19c[D85NH115N]

    Plasmid

    #112247

    Purpose

    expression of fly mutant PP4

    Depositor

    Simone Di Giovanni

    Insert

    PP4 (Pp4-19C Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D85NH115N

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMSCV_puro_41589

    Plasmid

    #111632

    Purpose

    Complementation of Dicer_KO mESCs with mutated human DICER isoform

    Depositor

    Constance Ciaudo

    Insert

    FLAG-hsDicer (K70A) (DICER1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K70A

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TET-pLKO-neo NXPH4-sh3

    Plasmid

    #163746

    Purpose

    Lentivirus for inducible knockdown of NXPH4 (human)

    Depositor

    Roland Friedel

    Insert

    NXPH4 (NXPH4 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TET-pLKO-neo NXPH4-sh2

    Plasmid

    #163745

    Purpose

    Lentivirus for inducible knockdown of NXPH4 (human)

    Depositor

    Roland Friedel

    Insert

    NXPH4 (NXPH4 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TET-pLKO-neo NXPH4-sh1

    Plasmid

    #163744

    Purpose

    Lentivirus for inducible knockdown of NXPH4 (human)

    Depositor

    Roland Friedel

    Insert

    NXPH4 (NXPH4 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge